Anti-Factor B抗体(ab72658)
Key features and details
- Rabbit polyclonal to Factor B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Factor B抗体
参阅全部 Factor B 一抗 -
描述
兔多克隆抗体to Factor B -
宿主
Rabbit -
特异性
ab72658 detects a fragment from activated Factor B; the Bb fragment, resulting from cleavage adjacent to Lys260. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Sheep, Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide:
KIVLDPSGSMN IYLVLDGSD
, corresponding to amino acids 260-280 of Human Factor B. -
阳性对照
- Extracts from K562 cells, treated with etoposide (25uM, 1hour).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72658于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 61 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 61 kDa). |
靶标
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功能
Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes. -
疾病相关
Defects in CFB are a cause of susceptibility to hemolytic uremic syndrome atypical type 4 (AHUS4) [MIM:612924]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
序列相似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 3 Sushi (CCP/SCR) domains.
Contains 1 VWFA domain. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 494140 Chimpanzee
- Entrez Gene: 629 Human
- Entrez Gene: 14962 Mouse
- Omim: 138470 Human
- SwissProt: Q864W0 Chimpanzee
- SwissProt: Q864V9 Gorilla
- SwissProt: P00751 Human
- SwissProt: P04186 Mouse
see all -
别名
- AHUS4 antibody
- B factor properdin antibody
- BF antibody
see all
图片
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All lanes : Anti-Factor B antibody (ab72658) at 1/200 dilution
Lane 1 : Extracts from K562 cells, treated with etoposide (25uM, 1hour)
Lane 2 : Extracts from K562 cells, treated with etoposide (25uM, 1hour) with Cl-peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 61 kDa
Observed band size: 54,86 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa (possible non-specific binding), 75 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab72658 被引用在 2 文献中.
- Shahulhameed S et al. A Systematic Investigation on Complement Pathway Activation in Diabetic Retinopathy. Front Immunol 11:154 (2020). PubMed: 32117292
- Bongoni AK et al. Activation of the lectin pathway of complement in pig-to-human xenotransplantation models. Transplantation 96:791-9 (2013). PubMed: 23958924