概述

  • 产品名称Anti-FACL4抗体
    参阅全部 FACL4 一抗
  • 描述
    兔多克隆抗体to FACL4
  • 经测试应用适用于: ELISA, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide (10-30 aa in length) in the region of 276~292 of human FACL4.

  • 阳性对照
    • RKO cell lysate, hepatocarcinoma tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

性能

应用

Our Abpromise guarantee covers the use of ab38420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 79 kDa.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.
  • 疾病相关Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
    Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis.
  • 序列相似性Belongs to the ATP-dependent AMP-binding enzyme family.
  • 细胞定位Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACS 4 antibody
    • ACS4 antibody
    • ACSL 4 antibody
    • Acsl4 antibody
    • ACSL4_HUMAN antibody
    • acyl CoA synthetase 4 antibody
    • Acyl CoA synthetase long chain family member 4 antibody
    • FACL 4 antibody
    • FACL4 antibody
    • Fatty acid Coenzyme A ligase antibody
    • fatty acid Coenzyme A ligase long-chain 4 antibody
    • LACS 4 antibody
    • LACS4 antibody
    • Lignoceroyl CoA synthase antibody
    • Long chain 4 antibody
    • long chain acyl CoA synthetase 4 antibody
    • long chain fatty acid CoA ligase 4 antibody
    • long chain fatty acid Coenzyme A ligase 4 antibody
    • Long-chain acyl-CoA synthetase 4 antibody
    • Long-chain-fatty-acid--CoA ligase 4 antibody
    • MRX63 antibody
    • MRX68 antibody
    see all

Anti-FACL4 antibody 图像

  • ICC/IF image of ab38420 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab38420 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-FACL4 antibody (ab38420) + RKO cell lysate (human colon cancer) treated with 10% FCS for 24hrs

    Predicted band size : 79 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of Drs. M.G. Sciulli and P. Patrignani, University of Chieti

  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-FACL4 antibody (ab38420)参考文献

ab38420 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab38420.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"