概述

  • 产品名称Anti-FABP4抗体
    参阅全部 FABP4 一抗
  • 描述
    兔多克隆抗体to FABP4
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human FABP4.

    (Peptide available as ab66681.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Human Adipose Tissue, P7 Mouse Adipose Tissue, Rat Brown Adipose Tissue, Mouse Thymus Tissue, Rat Ovary Tissue, Rat Colon Tissue, Rat Thymus Tissue, HepG2 Whole Cell, HeLa Whole Cell

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应用

Our Abpromise guarantee covers the use of ab66682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).Can be blocked with Human FABP4 peptide (ab66681).

靶标

  • 功能Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
  • 序列相似性Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • 结构域Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • 细胞定位Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3T3-L1 lipid-binding protein antibody
    • 422/aP2 antibody
    • A-FABP antibody
    • adipocyte antibody
    • Adipocyte lipid binding protein antibody
    • Adipocyte lipid-binding protein antibody
    • Adipocyte protein AP2 antibody
    • Adipocyte-type fatty acid-binding protein antibody
    • AFABP antibody
    • ALBP antibody
    • ALBP/Ap2 antibody
    • aP2 antibody
    • Epididymis secretory protein Li 104 antibody
    • FABP antibody
    • FABP4 antibody
    • FABP4_HUMAN antibody
    • Fatty acid binding protein 4 adipocyte antibody
    • Fatty acid binding protein 4 antibody
    • Fatty acid binding protein adipocyte antibody
    • Fatty acid-binding protein 4 antibody
    • Fatty acid-binding protein antibody
    • HEL S 104 antibody
    • Lbpl antibody
    • Myelin P2 protein homolog antibody
    • P15 antibody
    • P2 adipocyte protein antibody
    • Protein 422 antibody
    see all

Anti-FABP4 antibody 图像

  • All lanes : Anti-FABP4 antibody (ab66682) at 1 µg/ml

    Lane 1 : Human adipose normal tissue lysate - total protein (ab28980)
    Lane 2 : P7 Mouse Adipose Tissue Lysate
    Lane 3 : Adult Rat Brown Adipose Tissue Lysate (ab48639)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
    Additional bands at : 30 kDa,44 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab66682 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • All lanes : Anti-FABP4 antibody (ab66682) at 1 µg/ml

    Lane 1 : Mouse skeletal muscle tissue lysate - total protein (ab29711)
    Lane 2 : Mouse thymus tissue lysate - total protein (ab29285)
    Lane 3 : Rat Ovary Tissue Lysate
    Lane 4 : Rat Colon Tissue Lysate
    Lane 5 : Rat Thymus Tissue Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 15 kDa
    Observed band size : 15 kDa
    Additional bands at : 18 kDa (possible post-translational modification).

Anti-FABP4 antibody (ab66682)参考文献

This product has been referenced in:
  • Roberts JN  et al. Dynamic Surfaces for the Study of Mesenchymal Stem Cell Growth through Adhesion Regulation. ACS Nano 10:6667-79 (2016). ICC/IF . Read more (PubMed: 27322014) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Sample Human Cell (Adipose-derived stem cells for adipogenesis)
Specification Adipose-derived stem cells for adipogenesis
Permeabilization Yes - 0.5% Triton X-100, room temperature 15 mintutes
Fixative Paraformaldehyde
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提交于 Mar 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"