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Our Abpromise guarantee covers the use of ab23693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 4 µg/ml.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).|
ab23693 staining FABP4 (pink) in human adipose-derived stem cells for adipogenesis by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes and blocked with 10% serum for 1 hour at 37°C. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Green - Phalloidin, Blue - DAPI.
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