概述

  • 产品名称
  • 描述
    兔多克隆抗体to FABP4
  • 经测试应用
    适用于: WB, ELISA, IHC-P, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein (Human).

性能

应用

Our Abpromise guarantee covers the use of ab13979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 15 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/200.
IP Use at an assay dependent concentration.

靶标

  • 功能
    Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
  • 序列相似性
    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • 结构域
    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • 细胞定位
    Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3T3-L1 lipid-binding protein antibody
    • 422/aP2 antibody
    • A-FABP antibody
    • adipocyte antibody
    • Adipocyte lipid binding protein antibody
    • Adipocyte lipid-binding protein antibody
    • Adipocyte protein AP2 antibody
    • Adipocyte-type fatty acid-binding protein antibody
    • AFABP antibody
    • ALBP antibody
    • ALBP/Ap2 antibody
    • aP2 antibody
    • Epididymis secretory protein Li 104 antibody
    • FABP antibody
    • FABP4 antibody
    • FABP4_HUMAN antibody
    • Fatty acid binding protein 4 adipocyte antibody
    • Fatty acid binding protein 4 antibody
    • Fatty acid binding protein adipocyte antibody
    • Fatty acid-binding protein 4 antibody
    • Fatty acid-binding protein antibody
    • HEL S 104 antibody
    • Lbpl antibody
    • Myelin P2 protein homolog antibody
    • P15 antibody
    • P2 adipocyte protein antibody
    • Protein 422 antibody
    see all

Anti-FABP4 antibody 图像

  • Immunohistochemical analysis of FFPE human adipose tissue, labeling FABP4 with ab13979 (2µg/ml). DAB staining.

  • Immunohistochemical analysis of Human carotid atherosclerotic plaques, staining FABP4 with ab13979.

    Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6) and blocked with 0.5% BSA. Sections were incubated with primary antibody for 1 hour at room temperature. Staining was detected using DAB.
  • Immunohistochemical analysis of FFPE mouse pancreatic and peri-pancreatic fatty tissue, labeling FABP4 with ab13979. Tissue was fixed in 10% neutral buffered formalin, heat mediated antigen retrieval was performed using citrate buffer, and blocking was performed with 10% goat serum and 1% BSA inj TBST for 1 hour at 25°C. 

    Staining with ab13979 (diluted 1/500) was performed for 12 hours at 4°C. A goat anti-rabbit HRP-conjugated secondary was used, diluted 1/200.

    See Abreview

  • Immunohistochemical analysis of Human carotid atherosclerotic plaques, staining FABP4 with ab13979.

    Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6) and blocked with 0.5% BSA. Sections were incubated with primary antibody for 1 hour at room temperature. An AlexaFluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Anti-FABP4 antibody (ab13979)参考文献

This product has been referenced in:
  • Gunnar E  et al. sequoia controls the type I>0 daughter proliferation switch in the developing Drosophila nervous system. Development 143:3774-3784 (2016). Read more (PubMed: 27578794) »
  • Su X  et al. Expression of FABP4, adipsin and adiponectin in Paneth cells is modulated by gut Lactobacillus. Sci Rep 5:18588 (2015). Read more (PubMed: 26687459) »

See all 9 Publications for this product

Product Wall

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The antibody you have ordered is a rabbit polyclonal to FABP4 therefore, you will need to use an anti-rabbit secondary antibody so that it can recognise and bind to your primary antibody.

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Thank you for contacting Abcam regarding ab13979.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

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Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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Thank you for your enquiry. The immunogen used to make this antibody was not a peptide but the full length protein. SwissProt link (http://www.uniprot.org/uniprot/P15090) I have taken the liberty BLASTing the sequence with zebrafish to check f...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas and peri-pancreatic fatty tissue)
Specification
pancreas and peri-pancreatic fatty tissue
Fixative
10% NBF
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Blocking step
10% goat serum/1%BSA in TBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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提交于 Aug 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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