Anti-FABP4抗体[9B8D] (ab93945)

概述

  • 产品名称Anti-FABP4抗体[9B8D]
    参阅全部 FABP4 一抗
  • 描述
    小鼠单克隆抗体[9B8D] to FABP4
  • 经测试应用适用于: WB, ELISA, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified recombinant fragment of Human FABP4 expressed in E. coli.

  • 阳性对照
    • FABP4-hIgGFc transfected HEK293 cell lysate

应用

Our Abpromise guarantee covers the use of ab93945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
ICC/IF
Flow Cyt
  • 应用说明ELISA: 1/10,000.
    ICC/IF: 1/200 - 1/1000.
    WB: 1/500 - 1/2000. Predicted molecular weight: 15 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
    • 序列相似性Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
    • 结构域Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
    • 细胞定位Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 3T3-L1 lipid-binding protein antibody
      • 422/aP2 antibody
      • A-FABP antibody
      • adipocyte antibody
      • Adipocyte lipid binding protein antibody
      • Adipocyte lipid-binding protein antibody
      • Adipocyte protein AP2 antibody
      • Adipocyte-type fatty acid-binding protein antibody
      • AFABP antibody
      • ALBP antibody
      • ALBP/Ap2 antibody
      • aP2 antibody
      • Epididymis secretory protein Li 104 antibody
      • FABP antibody
      • FABP4 antibody
      • FABP4_HUMAN antibody
      • Fatty acid binding protein 4 adipocyte antibody
      • Fatty acid binding protein 4 antibody
      • Fatty acid binding protein adipocyte antibody
      • Fatty acid-binding protein 4 antibody
      • Fatty acid-binding protein antibody
      • HEL S 104 antibody
      • Lbpl antibody
      • Myelin P2 protein homolog antibody
      • P15 antibody
      • P2 adipocyte protein antibody
      • Protein 422 antibody
      see all

    Anti-FABP4 antibody [9B8D] 图像

    • Overlay histogram showing THP1 cells stained with ab93945 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93945, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Anti-FABP4 antibody [9B8D] (ab93945) at 1/500 dilution + FABP4-hIgGFc transfected HEK293 cell lysate

      Predicted band size : 15 kDa

    Anti-FABP4 antibody [9B8D] (ab93945)参考文献

    ab93945 has not yet been referenced specifically in any publications.

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