概述

  • 产品名称Anti-FA2H抗体
    参阅全部 FA2H 一抗
  • 描述
    小鼠多克隆抗体to FA2H
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human FA2H protein (AAH17049.2)

  • 阳性对照
    • FA2H transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab68902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 43 kDa).

靶标

  • 功能Required for alpha-hydroxylation of free fatty acids and the formation of alpha-hydroxylated sphingolipids.
  • 组织特异性Detected in differentiating cultured keratinocytes (at protein level). Detected in epidermis and cultured keratinocytes. Highly expressed in brain and colon. Detected at lower levels in testis, prostate, pancreas and kidney.
  • 疾病相关Defects in FA2H are the cause of leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia (DLDSP) [MIM:612443]. The disorder consists of a progressive neurologic disease manifested by spasticity, disordered tonicity of muscle, and white matter degeneration.
    Defects in FA2H are a cause of spastic paraplegia autosomal recessive type 35 (SPG35) [MIM:612319]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
  • 序列相似性Belongs to the sterol desaturase family. SCS7 subfamily.
    Contains 1 cytochrome b5 heme-binding domain.
  • 结构域The histidine box domains may contain the active site and/or be involved in metal ion binding.
  • 细胞定位Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FA2H antibody
    • FA2H_HUMAN antibody
    • FAAH antibody
    • FAH1 antibody
    • Fatty acid 2 hydroxylase antibody
    • Fatty acid 2-hydroxylase antibody
    • Fatty acid alpha hydroxylase antibody
    • Fatty acid alpha-hydroxylase antibody
    • Fatty acid hydroxylase domain containing 1 antibody
    • FAXDC1 antibody
    • FLJ25287 antibody
    • SCS7 antibody
    • Spastic paraplegia 35 (autosomal recessive) antibody
    • SPG35 antibody
    see all

Anti-FA2H antibody 图像

  • All lanes : Anti-FA2H antibody (ab68902) at 1/500 dilution

    Lane 1 : FA2H transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 43 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

Anti-FA2H antibody (ab68902)参考文献

This product has been referenced in:
  • Palumbo-Zerr K  et al. Inactivation of fatty acid amide hydrolase exacerbates experimental fibrosis by enhanced endocannabinoid-mediated activation of CB1. Ann Rheum Dis 71:2051-4 (2012). Read more (PubMed: 22915616) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hamster CHO cells transfected with human FA2H cDNA)
Specification Hamster CHO cells transfected with human FA2H cDNA
Fixative Formaldehyde
Permeabilization Yes - 0.5% Nonidet P-40
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
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提交于 Apr 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"