概述

  • 产品名称Anti-F4/80抗体[CI:A3-1]
    参阅全部 F4/80 一抗
  • 描述
    大鼠单克隆抗体[CI:A3-1] to F4/80
  • 特异性This antibody recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages. Abcam does not recommend this product for IHC-P.
  • 经测试应用适用于: WB, IP, RIA, IHC-Fr, Flow Cyt, ICC, IHC-FoFr, ICC/IF, IHC-Rmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Thioglycollate stimulated peritoneal macrophages from C57/BL mice

  • 阳性对照
    • Mouse spleen and macrophages ICC/IF: Raw264.7 cells.
  • 常规说明

    Please note we cannot guarantee IHC-P, however should you use this application for this product then please use a specific protocol which can be found in the protocols section of our datasheet here.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.60
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.164% Sodium phosphate, 1.45% Sodium chloride, 1% BSA
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • 克隆单克隆
  • 克隆编号CI:A3-1
  • 骨髓瘤NS1
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 10 µg/ml. Use under non reducing condition. Detects a band of approximately 160 kDa (predicted molecular weight: 102 kDa). (predicted molecular weight of precursor protein: 102 kDa; protein is heavily glycosylated). Block in 5% milk for 1 hour.
IP Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-Fr Use a concentration of 10 µg/ml.
Flow Cyt 1/10 - 1/50.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ICC Use at an assay dependent concentration.
IHC-FoFr Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 5 - 10 µg/ml.
IHC-R Use a concentration of 10 µg/ml.

靶标

  • 功能Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • 组织特异性Expression is restricted to eosinophils.
  • 序列相似性Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Anti-F4/80 antibody [CI:A3-1] 图像

  • ab6640 stained Raw 264.7 cells. The cells were 100% methanol fixed for 5 minutes at room temperatureand then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6640 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-F4/80 antibody [CI:A3-1] (ab6640) at 1/1000 dilution

    Lane 1 : Mouse macrophage lysates
    Lane 2 : Mouse hepatocyte lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP labelled donkey anti-rat at 1/5000 dilution

    Predicted band size : 102 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab6640 staining F4/80 in mouse spleen tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and blocked with 5% serum for 45 minutes at 21°C. The sample was incubated with primary antibody (1/100 in PBS + 5% FBS) at 4°C for 16 hours. An Alexa Fluor® 488-conjugated goat anti-rat IgG (H+L) polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab6640 staining F4/80 in mouse bone marrow cells by immunocytochemistry/ immunofluorescence. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hour at 4°C. Alexa fluor® 488 goat polyclonal to rat Ig, diluted 1/200, was used as the secondary antibody.

    See Abreview

  • Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/10 followed by streptavidin FITC secondary at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control. 

  • Flow cytometry analysis of peritoneal macrophages labelling F4/80 (red) with ab6640 at a dilution of 1/10. The blue line shows J774 cells stained with Rat anti mouse isotype control. 

  • Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/50 followed by goat anti rat IgG FITC secondary antibody at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control. 

  • ab6640 staining F4/80 (red) in Mouse intestine tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 1 hour at 21°C. Samples were incubated with primary antibody (1/10µg/ml in PBS + 10% serum) for 16 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rat IgG polyclonal (1/1000) was used as the secondary antibody. Blue - nuclei.

    See Abreview

Anti-F4/80 antibody [CI:A3-1] (ab6640)参考文献

This product has been referenced in:
  • Doi H  et al. Potency of umbilical cord blood- and Wharton's jelly-derived mesenchymal stem cells for scarless wound healing. Sci Rep 6:18844 (2016). IHC ; Mouse . Read more (PubMed: 26728342) »
  • Shiokawa M  et al. Pathogenicity of IgG in patients with IgG4-related disease. Gut N/A:N/A (2016). Human . Read more (PubMed: 26964842) »

See all 129 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Embryonic Heart)
Permeabilization Yes - Tween-20
Specification Embryonic Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Verified customer

提交于 Oct 11 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 30 µg
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 04 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrite buffer, pH6.0
Permeabilization No
Specification spleen
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
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提交于 Aug 19 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Jaculus jaculus Tissue sections (spleen)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: proteaseK
Permeabilization No
Specification spleen
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Mai Tran

Verified customer

提交于 Jul 12 2016

Application Western blot
Sample Human Cell lysate - whole cell (Human Macrophages)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 30 µg
Specification Human Macrophages
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

提交于 Apr 06 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH=6
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative 10% formalin
Username

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提交于 Feb 18 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer 1X
Permeabilization No
Specification kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Formaldehyde
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提交于 Oct 14 2015

Application Immunocytochemistry
Sample Mouse Cultured Cells (peritoneal macrophages)
Permeabilization No
Specification peritoneal macrophages
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
Username

Dr. Varkha Agrawal

Verified customer

提交于 Jun 05 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Antigen retrieval step None
Sample Mouse Tissue sections (Spleen)
Specification Spleen
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 05 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (hepatocytes)
Specification hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

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提交于 Dec 30 2014

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