Anti-F4/80抗体[BM8] (FITC) (ab60343)

概述

  • 产品名称Anti-F4/80抗体[BM8] (FITC)
    参阅全部 F4/80 一抗
  • 描述
    大鼠单克隆抗体[BM8] to F4/80 (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性This antibody reacts with a 125kDa extracellular macrophage membrane molecule, highly restricted to mature macrophage subpopulations residing in tissue. It does not cross react with any of the following mouse cell types: Granulocytes, mast cells, platelets, lymophocytes, fibroblasts or endothelial cells. Although several publications have used this antibody succesfully in human, we have been unable to obtain positive results in this species and so do not guarantee it.
  • 经测试应用适用于: WB, IHC-Fr, Flow Cyt, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    BALB/c macrophages obtained from 14-day-old bone marrow cell cultures.

  • 阳性对照
    • Mouse macrophages.
  • 常规说明


    This antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. It is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 纯化说明Purity: 0.2µm filtered.
  • Primary antibody说明This antibody is the only macrophage marker that is able to distinguish non-destructive from destructive inflammation processes in the pancreas. It is a unique histological marker of the progression from peri-insulitis to beta-cell destruction and diabetes in a mouse diabetes model.
  • 克隆单克隆
  • 克隆编号BM8
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab60343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50. Predicted molecular weight: 98 kDa.
IHC-Fr 1/50. tissue embedded in OCT Tissue Tec; fixed with acetone for 10 min at RT; incubation with 0.02 M sodium azide in PBS containing 0.1 % H2O2 for 10 min at RT to destroy endogenous peroxidase; spleen as positive control.
Flow Cyt 1/50 - 1/100. ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-P 1/50. fixation in 10% neutral buffered formalin for 24 h; blocking with non-immunized goat serum; microwaved for 6 min in citrate buffer; splenic macrophages as positive control.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • 组织特异性Expression is restricted to eosinophils.
  • 序列相似性Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Anti-F4/80 antibody [BM8] (FITC) 图像

  • ICC/IF image of ab60343 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60343, 1µg/ml, FITC conjugated) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing RAW 264.7 cells stained with ab60343 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab60343, 1/100 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) with secondary antibody DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Acquisition of >5,000 events was performed. This antibody gave a minimal signal in RAW 264.7 cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-F4/80 antibody [BM8] (FITC) (ab60343)参考文献

This product has been referenced in:
  • Balamurugan K  et al. FBXW7a attenuates inflammatory signalling by downregulating C/EBPd and its target gene Tlr4. Nat Commun 4:1662 (2013). Read more (PubMed: 23575666) »
  • Majumder M  et al. A practical and sensitive method of quantitating lymphangiogenesis in vivo. Lab Invest 93:779-91 (2013). Mouse . Read more (PubMed: 23711825) »

See all 8 Publications for this product

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Antibody ab60343 is a well-characterized F4/80 clone, BM8. If the cells express F4/80, the antibody will detect it. For more references, see the datasheet for the unconjugated version, ab16911:

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