概述

  • 产品名称Anti-F4/80抗体
    参阅全部 F4/80 一抗
  • 描述
    兔多克隆抗体to F4/80
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    A synthetic peptide derived from N-terminus of Mouse F4/80.

  • 阳性对照
    • Human lung ICC: RAW246.7 cell line

性能

应用

Our Abpromise guarantee covers the use of ab100790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100.

Boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min.  This product may benefit from the use of freshly cut tissue. 

ICC/IF 1/100.

靶标

  • 功能Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • 组织特异性Expression is restricted to eosinophils.
  • 序列相似性Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

Anti-F4/80 antibody 图像

  • ab100790 stained RAW 246.7 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (abxxx at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Staining of F4/80 in a Formalin/PFA-fixed paraffin-embedded section of human lung using ab100790 at a dilution of 1/100.

  • Staining of F4/80 in a Formalin/PFA-fixed paraffin-embedded section of human lung using ab100790 at a dilution of 1/100.

Anti-F4/80 antibody (ab100790)参考文献

This product has been referenced in:
  • Velsko IM  et al. Active invasion of oral and aortic tissues by Porphyromonas gingivalis in mice causally links periodontitis and atherosclerosis. PLoS One 9:e97811 (2014). IHC-P ; Mouse . Read more (PubMed: 24836175) »
  • Serpooshan V  et al. The effect of bioengineered acellular collagen patch on cardiac remodeling and ventricular function post myocardial infarction. Biomaterials 34:9048-55 (2013). Mouse . Read more (PubMed: 23992980) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (xenograft tissue)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 9 access super buffer
Permeabilization No
Specification xenograft tissue
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative Formaldehyde
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提交于 Nov 13 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"