Anti-F4/80抗体(ab100790)
Key features and details
- Rabbit polyclonal to F4/80
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-F4/80抗体
参阅全部 F4/80 一抗 -
描述
兔多克隆抗体to F4/80 -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse
预测可用于: Rat不与反应: Human -
免疫原
Synthetic peptide within Mouse F4/80 aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: Q61549 -
阳性对照
- IHC-P: Mouse Liver tissue
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab100790于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (4) |
1/100.
Boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min. This product may benefit from the use of freshly cut tissue. |
说明 |
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IHC-P
1/100. Boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min. This product may benefit from the use of freshly cut tissue. |
靶标
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功能
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
组织特异性
Expression is restricted to eosinophils. -
序列相似性
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 13733 Mouse
- Entrez Gene: 316137 Rat
- SwissProt: Q61549 Mouse
- SwissProt: Q5Y4N8 Rat
- Unigene: 2254 Mouse
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别名
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
图片
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IHC image of F4/80 staining in a section of formalin-fixed paraffin-embedded normal mouse liver performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab100790, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (84)
ab100790 被引用在 84 文献中.
- Du M et al. The role of long noncoding RNA Nron in atherosclerosis development and plaque stability. iScience 25:103978 (2022). PubMed: 35295812
- Chen C et al. Engineering an enthesis-like graft for rotator cuff repair: An approach to fabricate highly biomimetic scaffold capable of zone-specifically releasing stem cell differentiation inducers. Bioact Mater 16:451-471 (2022). PubMed: 35386315
- Araujo N et al. Tumor Suppressor Par-4 Regulates Complement Factor C3 and Obesity. Front Oncol 12:860446 (2022). PubMed: 35425699
- Srivastava RK et al. Role of hair follicles in the pathogenesis of arsenical-induced cutaneous damage. Ann N Y Acad Sci 1515:168-183 (2022). PubMed: 35678766
- Zhu Q et al. N-acetylcysteine attenuates atherosclerosis progression in aging LDL receptor deficient mice with preserved M2 macrophages and increased CD146. Atherosclerosis 357:41-50 (2022). PubMed: 36041358