Anti-F box蛋白38抗体(ab87729)

概述

  • 产品名称
    Anti-F box蛋白38抗体
  • 描述
    兔多克隆抗体to F box蛋白38
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 825 and 875 of Human F box protein 38 (NP_995308.1).

  • 阳性对照
    • HeLa and NIH3T3 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab87729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 134 kDa.
IP Use at 5-10 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (right) and human testicular seminoma (right) tissues labelling F box protein 38 with ab87729 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-F box protein 38 antibody (ab87729) at 0.1 µg/ml

    Lane 1 : Whole cell
    lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell
    lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell
    lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell
    lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 134 kDa
    Observed band size: 134 kDa
    Additional bands at: 107 kDa (possible isoform)


    Exposure time: 3 minutes
  • Detection of F box protein 38 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab87729 at 10 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

文献

ab87729 has not yet been referenced specifically in any publications.

客户评价及客户问答

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