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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558). A synthetic peptide corresponding to residues surrounding Thr567 (Ezrin), Thr564 (Radixin) and Thr558 (Moesin) of human Ezrin.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
This antibody detects the phosphorylated target and not the non-phosphorylated epitope.
Our Abpromise guarantee covers the use of ab76247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 75-81 kDa (predicted molecular weight: 69 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma with ab76247 at 1/100 dilution.
Dot blot analysis of Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) phospho peptide (Lane 1), Non-phospho peptide (Lane 2), labelling Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) with ab76247 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
The sequence of non-phospho peptide: CGRDKYKTLRQIR.
The sequence of phospho peptide: CGRDKYK-pT-LRQIR.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"