Anti-Ezrin (phospho Y478)抗体(ab79256)

概述

  • 产品名称Anti-Ezrin (phospho Y478)抗体
    参阅全部 Ezrin 一抗
  • 描述
    兔多克隆抗体to Ezrin (phospho Y478)
  • 特异性Binds to Ezrin only when phosphorylated at tyrosine 478.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human Ezrin around the phosphorylation site of tyrosine 478 (P-V-YP-E-P)

  • 阳性对照
    • Extracts form HeLa cells treated with Na3VO4 (0.3nM, 40mins).

性能

应用

Our Abpromise guarantee covers the use of ab79256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 69 kDa.
ELISA 1/5000.

靶标

  • 功能Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
  • 组织特异性Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
  • 序列相似性Contains 1 FERM domain.
  • 发展阶段Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
  • 翻译后修饰Phosphorylated by tyrosine-protein kinases.
  • 细胞定位Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Villin 2 ezrin antibody
    • CVIL antibody
    • CVL antibody
    • Cytovillin 2 antibody
    • Cytovillin antibody
    • DKFZp762H157 antibody
    • Epididymis secretory protein Li 105 antibody
    • EZR antibody
    • EZRI_HUMAN antibody
    • Ezrin antibody
    • FLJ26216 antibody
    • HEL S 105 antibody
    • MGC1584 antibody
    • p81 antibody
    • VIL 2 antibody
    • VIL2 antibody
    • Villin 2 (ezrin) antibody
    • Villin 2 antibody
    • Villin-2 antibody
    • Villin2 antibody
    see all

Anti-Ezrin (phospho Y478) antibody 图像

  • All lanes : Anti-Ezrin (phospho Y478) antibody (ab79256) at 1/500 dilution

    Lane 1 : K562 cell lysate treated with Na3VO4 (0.3nM, 40mins)
    Lane 2 : K562 cell lysate treated with Na3VO4 (0.3nM, 40mins) with immunizing phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 69 kDa
    Observed band size : 69 kDa

Anti-Ezrin (phospho Y478) antibody (ab79256)参考文献

ab79256 has not yet been referenced specifically in any publications.

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