Anti-Ezrin (phospho Y353)抗体[EP925Y] (ab52916)


  • 产品名称Anti-Ezrin (phospho Y353)抗体[EP925Y]
    参阅全部 Ezrin 一抗
  • 描述
    兔单克隆抗体[EP925Y] to Ezrin (phospho Y353)
  • 特异性Detects Ezrin phosphorylated on Tyrosine 353.
  • 经测试应用适用于: WBmore details
    不适用于: ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phospho-specific peptide corresponding to residues surrounding Tyr353P of human Ezrin.

  • 阳性对照
    • Lysate of 293 cells treated with pervanadate.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab52916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200000. Predicted molecular weight: 69 kDa.
  • 应用说明Is unsuitable for ICC/IF,IHC-P or IP.
  • 靶标

    • 功能Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
    • 组织特异性Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
    • 序列相似性Contains 1 FERM domain.
    • 发展阶段Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
    • 翻译后修饰Phosphorylated by tyrosine-protein kinases.
    • 细胞定位Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Villin 2 ezrin antibody
      • CVIL antibody
      • CVL antibody
      • Cytovillin 2 antibody
      • Cytovillin antibody
      • DKFZp762H157 antibody
      • Epididymis secretory protein Li 105 antibody
      • EZR antibody
      • EZRI_HUMAN antibody
      • Ezrin antibody
      • FLJ26216 antibody
      • HEL S 105 antibody
      • MGC1584 antibody
      • p81 antibody
      • VIL 2 antibody
      • VIL2 antibody
      • Villin 2 (ezrin) antibody
      • Villin 2 antibody
      • Villin-2 antibody
      • Villin2 antibody
      see all

    Anti-Ezrin (phospho Y353) antibody [EP925Y] 图像

    • All lanes : Anti-Ezrin (phospho Y353) antibody [EP925Y] (ab52916) at 1/200000 dilution

      Lane 1 : 293 cell lysate untreated
      Lane 2 : 293 cell lysate treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Predicted band size : 69 kDa
      Observed band size : 69 kDa

    Anti-Ezrin (phospho Y353) antibody [EP925Y] (ab52916)参考文献

    ab52916 has not yet been referenced specifically in any publications.

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