Anti-Ezrin (phospho Y146)抗体(ab92557)

概述

  • 产品名称Anti-Ezrin (phospho Y146)抗体
    参阅全部 Ezrin 一抗
  • 描述
    兔多克隆抗体to Ezrin (phospho Y146)
  • 特异性Detects endogenous levels of Ezrin only when phosphorylated at Tyrosine 146.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human Ezrin around the phosphorylation site of Tyrosine 146 (SGYPLS).

  • 阳性对照
    • Extracts from UV-treated 293 cells

性能

应用

Our Abpromise guarantee covers the use of ab92557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: 1/40000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 69 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
    • 组织特异性Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
    • 序列相似性Contains 1 FERM domain.
    • 发展阶段Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
    • 翻译后修饰Phosphorylated by tyrosine-protein kinases.
    • 细胞定位Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Villin 2 ezrin antibody
      • CVIL antibody
      • CVL antibody
      • Cytovillin 2 antibody
      • Cytovillin antibody
      • DKFZp762H157 antibody
      • Epididymis secretory protein Li 105 antibody
      • EZR antibody
      • EZRI_HUMAN antibody
      • Ezrin antibody
      • FLJ26216 antibody
      • HEL S 105 antibody
      • MGC1584 antibody
      • p81 antibody
      • VIL 2 antibody
      • VIL2 antibody
      • Villin 2 (ezrin) antibody
      • Villin 2 antibody
      • Villin-2 antibody
      • Villin2 antibody
      see all

    Anti-Ezrin (phospho Y146) antibody 图像

    • All lanes : Anti-Ezrin (phospho Y146) antibody (ab92557) at 1/500 dilution

      Lane 1 : 293 cells (treated with UV for 15 mins)
      Lane 2 : 293 cells (treated with UV for 15 mins) with immunizing peptide at 5 µg

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 69 kDa

    Anti-Ezrin (phospho Y146) antibody (ab92557)参考文献

    ab92557 has not yet been referenced specifically in any publications.

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