概述

  • 产品名称Anti-Ezrin (phospho T567)抗体
    参阅全部 Ezrin 一抗
  • 描述
    兔多克隆抗体to Ezrin (phospho T567)
  • 特异性This antibody detects endogenous levels of Ezrin only when phosphorylated at Threonine 567. This antibody will also react with Moesin at Threonine 577 and Radixin at Threonine 564.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Ssynthesized phosphopeptide derived from human Ezrin around the phosphorylation site of threonine 567 (Y-K-TP-L-R)

  • 阳性对照
    • Extracts from A431 cells.

性能

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应用

Our Abpromise guarantee covers the use of ab47293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
ELISA 1/20000.
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
  • 组织特异性Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
  • 序列相似性Contains 1 FERM domain.
  • 发展阶段Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
  • 翻译后修饰Phosphorylated by tyrosine-protein kinases.
  • 细胞定位Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Villin 2 ezrin antibody
    • CVIL antibody
    • CVL antibody
    • Cytovillin 2 antibody
    • Cytovillin antibody
    • DKFZp762H157 antibody
    • Epididymis secretory protein Li 105 antibody
    • EZR antibody
    • EZRI_HUMAN antibody
    • Ezrin antibody
    • FLJ26216 antibody
    • HEL S 105 antibody
    • MGC1584 antibody
    • p81 antibody
    • VIL 2 antibody
    • VIL2 antibody
    • Villin 2 (ezrin) antibody
    • Villin 2 antibody
    • Villin-2 antibody
    • Villin2 antibody
    see all

Anti-Ezrin (phospho T567) antibody 图像

  • All lanes : Anti-Ezrin (phospho T567) antibody (ab47293)

    Lane 1 : EGF treated A431 cells, no added peptide
    Lane 2 : Untreated A431 cells, no added peptide


    Predicted band size : 69 kDa
    Observed band size : 69 kDa
  • Ab47293 staining human normal placenta. Staining is localised to apical membrane and cytoplasmic compartiment.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • All lanes : Anti-Ezrin (phospho T567) antibody (ab47293)

    Lane 1 : Jurkat cell lysate with PMA (125 ng/ml, 30 minutes)
    Lane 2 : Jurkat cell lysate


    Predicted band size : 69 kDa
    Observed band size : 69 kDa
    Additional bands at : 68 kDa (possible cross reactivity).

Anti-Ezrin (phospho T567) antibody (ab47293)参考文献

This product has been referenced in:
  • Wang Y  et al. Akt/Ezrin Tyr353/NF-?B pathway regulates EGF-induced EMT and metastasis in tongue squamous cell carcinoma. Br J Cancer 110:695-705 (2014). Read more (PubMed: 24346284) »
  • Xiao Y  et al. Increased phosphorylation of ezrin is associated with the migration and invasion of fibroblast-like synoviocytes from patients with rheumatoid arthritis. Rheumatology (Oxford) N/A:N/A (2014). Human . Read more (PubMed: 24599913) »

See all 3 Publications for this product

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