Anti-Ezrin抗体(ab76872)

概述

  • 产品名称Anti-Ezrin抗体
    参阅全部 Ezrin 一抗
  • 描述
    小鼠单克隆抗体to Ezrin
  • 特异性Cross-reactivity with Radixin and Moesin has not been tested.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human Ezrin protein (AAH13903) with a 26 kDa tag.

  • 阳性对照
    • Immunogen, HepG2 cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab76872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 69 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
  • 组织特异性Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.
  • 序列相似性Contains 1 FERM domain.
  • 发展阶段Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain.
  • 翻译后修饰Phosphorylated by tyrosine-protein kinases.
  • 细胞定位Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Villin 2 ezrin antibody
    • CVIL antibody
    • CVL antibody
    • Cytovillin 2 antibody
    • Cytovillin antibody
    • DKFZp762H157 antibody
    • Epididymis secretory protein Li 105 antibody
    • EZR antibody
    • EZRI_HUMAN antibody
    • Ezrin antibody
    • FLJ26216 antibody
    • HEL S 105 antibody
    • MGC1584 antibody
    • p81 antibody
    • VIL 2 antibody
    • VIL2 antibody
    • Villin 2 (ezrin) antibody
    • Villin 2 antibody
    • Villin-2 antibody
    • Villin2 antibody
    see all

Anti-Ezrin antibody 图像

  • Anti-Ezrin antibody (ab76872) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 69 kDa
    Observed band size : 91 kDa (why is the actual band size different from the predicted?)
  • Anti-Ezrin antibody (ab76872) at 5 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 69 kDa
    Observed band size : 69 kDa

Anti-Ezrin antibody (ab76872)参考文献

ab76872 has not yet been referenced specifically in any publications.

Product Wall

The cross-reactivity has not been tested. As the epitope is undetermined it is hard to say whether there is cross -reactivity. Whole protein homologies: EZRIN MOESIN 73 % EZRIN RADIXIN 76 % MOESIN RADIXIN 81 %

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"