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Our Abpromise guarantee covers the use of ab4069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 22427845|
|WB||1/100 - 1/750. Predicted molecular weight: 69 kDa.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17311105|
|IHC-P||1/50 - 1/100.|
|IHC-Fr||1/50 - 1/100.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
This image is courtesy of an anonymous Abreview
ab4069 at 1/100 dilution staining human HeLa cells by ICC/IF. The cells were formaldehyde fixed and blocked with BSA/goat serum prior to incubation with the antibody for 30 minutes. An Alexa-Fluor ® 568 conjugated goat anti-mouse IgG was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"