概述

  • 产品名称
  • 描述
    山羊多克隆抗体to EZH1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow
  • 免疫原

    Synthetic peptide:

    EIPNPPTSKCITY

    , corresponding to amino acids 2-14 of Human EZH 1.

  • 阳性对照
    • MOLT 4 lysate.

性能

应用

Our Abpromise guarantee covers the use of ab3670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).Can be blocked with Human EZH1 peptide (ab22954).

靶标

  • 功能
    Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
  • 序列相似性
    Belongs to the histone-lysine methyltransferase family. EZ subfamily.
    Contains 1 SET domain.
  • 细胞定位
    Nucleus. Colocalizes with trimethylated 'Lys-27' of histone H3.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Enhancer of zeste homolog 1 (Drosophila) antibody
    • Enhancer of zeste homolog 1 antibody
    • ENX-2 antibody
    • ENX2 antibody
    • Ezh1 antibody
    • EZH1_HUMAN antibody
    • Histone-lysine N-methyltransferase EZH1 antibody
    see all

图片

  • Anti-EZH1 antibody (ab3670) at 2 µg/ml + MOLT4 cell lysate (in RIPA buffer) at 35 µg

    Predicted band size : 85 kDa
    Observed band size : 85 kDa

实验方案

文献

This product has been referenced in:
  • Luo L  et al. Niche astrocytes promote the survival, proliferation and neuronal differentiation of co-transplanted neural stem cells following ischemic stroke in rats. Exp Ther Med 13:645-650 (2017). Read more (PubMed: 28352345) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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