概述

  • 产品名称Anti-EZH1抗体
    参阅全部 EZH1 一抗
  • 描述
    兔多克隆抗体to EZH1
  • 经测试应用适用于: ChIP, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide CSNASASAVAETKEG (amino acids 372-386) of human EZH1

  • 阳性对照
    • Tested with human spleen lysate.

性能

应用

Our Abpromise guarantee covers the use of ab13665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration. PubMed: 23932971
ICC/IF Use at an assay dependent concentration. PubMed: 23932971
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa.

靶标

  • 功能Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
  • 序列相似性Belongs to the histone-lysine methyltransferase family. EZ subfamily.
    Contains 1 SET domain.
  • 细胞定位Nucleus. Colocalizes with trimethylated 'Lys-27' of histone H3.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Enhancer of zeste homolog 1 (Drosophila) antibody
    • Enhancer of zeste homolog 1 antibody
    • ENX-2 antibody
    • ENX2 antibody
    • Ezh1 antibody
    • EZH1_HUMAN antibody
    • Histone-lysine N-methyltransferase EZH1 antibody
    see all

Anti-EZH1 antibody 图像

  • Western blot detection of EZH1 in mouse (lane 1) and human (lane 2) spleen lysate using ab13665 at 2 ug/ml.

Anti-EZH1 antibody (ab13665)参考文献

This product has been referenced in:
  • Lee W  et al. PRC2 is recurrently inactivated through EED or SUZ12 loss in malignant peripheral nerve sheath tumors. Nat Genet 46:1227-32 (2014). Read more (PubMed: 25240281) »
  • Henriquez B  et al. Ezh1 and Ezh2 differentially regulate PSD-95 gene transcription in developing hippocampal neurons. Mol Cell Neurosci 57:130-43 (2013). WB, ChIP, ICC/IF ; Rat . Read more (PubMed: 23932971) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (cartilage)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: proteinase K 10ug/ml
Permeabilization No
Specification cartilage
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 24°C
Fixative Formaldehyde
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提交于 Aug 16 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HL60)
Loading amount 10 µg
Specification HL60
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

提交于 Jul 18 2007

Thank you for your enquiry. I have checked the database for information regarding this product, and it seems that we have made a mistake while uploading information on the datasheet. I can confirm that this product detects human and mouse samples an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"