The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/2000.
1/500 - 1/2000.
Use at 2-5 µg/mg of lysate.
应用说明Is unsuitable for WB.
功能Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity). May be involved in development of the eye.
序列相似性Belongs to the HAD-like hydrolase superfamily. EYA family.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR. Ser-266 phosphorylation is required for localization at sites of DNA damage and directing interaction with H2AX.
细胞定位Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs). With decreasing efficiency, translocalized to the nucleus by SIX2 and SIX5, and SIX4, respectively.