概述

  • 产品名称Anti-EYA3抗体
    参阅全部 EYA3 一抗
  • 描述
    兔多克隆抗体to EYA3
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Sheep, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to Human EYA3 aa 1-30.
    Database link: Q99504

  • 阳性对照
    • HeLa, 293T cell lysate, IHC controls:breast, colon and prostate carcinoma

性能

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应用

Our Abpromise guarantee covers the use of ab95876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 63 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/500.

靶标

  • 功能Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1, and seems to coactivate SIX2, SIX4 and SIX5. The repression of precursor cell proliferation in myoblasts by SIX1 is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex and seems to be dependent on EYA3 phosphatase activity (By similarity). May be involved in development of the eye.
  • 序列相似性Belongs to the HAD-like hydrolase superfamily. EYA family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR. Ser-266 phosphorylation is required for localization at sites of DNA damage and directing interaction with H2AX.
  • 细胞定位Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs). With decreasing efficiency, translocalized to the nucleus by SIX2 and SIX5, and SIX4, respectively.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI844637 antibody
    • DKFZp686C132 antibody
    • EYA3 antibody
    • EYA3_HUMAN antibody
    • Eyes absent 3 antibody
    • Eyes absent 3 homolog (Drosophila) antibody
    • Eyes absent homolog 3 antibody
    • OTTMUSP00000010370 antibody
    • RP11-460I13.4 antibody
    • RP23-442A20.1 antibody
    see all

Anti-EYA3 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling EYA3 with ab95876 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-EYA3 antibody (ab95876) at 0.04 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 63 kDa


    Exposure time : 30 seconds
  • Detection of human Eya3 by Western Blot of Immunoprecipitate. ab95876 at 0.04µg/ml staining Eya3 in HeLa cell lysate immunoprecipitated using ab95876 at 3µg/mg (1mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-EYA3 antibody (ab95876)参考文献

ab95876 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Sample Human Cell (Human Retinal Microvascular Enothelial)
Specification Human Retinal Microvascular Enothelial
Permeabilization Yes - 0.2% Triton X-100
Fixative Paraformaldehyde
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提交于 Jul 09 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"