Anti-EYA2抗体(ab95875)

概述

  • 产品名称Anti-EYA2抗体
    参阅全部 EYA2 一抗
  • 描述
    兔多克隆抗体to EYA2
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between residue 100 and 150 of human EYA2 (NP_005235.3)

  • 阳性对照
    • 293T cells lysate

性能

应用

Our Abpromise guarantee covers the use of ab95875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 59 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. Together with SIX1 and DACH2 seem to be involved in myogenesis. May be involved in development of the eye. Interaction with GNAZ and GNAI2 prevents nuclear translocation and transcriptional activity.
  • 组织特异性Highest expression in muscle with lower levels in kidney, placenta, pancreas, brain and heart.
  • 序列相似性Belongs to the HAD-like hydrolase superfamily. EYA family.
  • 发展阶段At the begin of fourth week of development detected in cytoplasm of somite cells. Between the sixth and eighth week of development detected in cytoplasm of limb bud cells.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EAB 1 antibody
    • EAB1 antibody
    • EYA 2 antibody
    • EYA2 antibody
    • EYA2_HUMAN antibody
    • eyes absent 2 homolog (Drosophila) antibody
    • Eyes absent homolog 2 (Drosophila) antibody
    • Eyes absent homolog 2 antibody
    • Translation of this uORF probably lowers the translation efficiency of EYA2 antibody
    see all

Anti-EYA2 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling EYA2 with ab95875 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-EYA2 antibody (ab95875) at 0.2 µg/ml

    Lane 1 : 293T cell lysate at 50 µg
    Lane 2 : 293T cell lysate at 15 µg
    Lane 3 : 293T cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 59 kDa


    Exposure time : 30 seconds
  • Detection of human Eya2 by Western Blot of Immunoprecipitate. ab95875 at 0.1µg/ml staining Eya2 in 293T cell lysate immunoprecipitated using ab95875 at 3µg/mg (1mg/IP; 20% of IP loaded/lane) Detection: Chemiluminescence with exposure time of 10 seconds..

Anti-EYA2 antibody (ab95875)参考文献

ab95875 has not yet been referenced specifically in any publications.

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