The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
Cadherins are calcium dependent cell adhesion proteins which mediate cell-to-cell interaction. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. EY cadherin (Cadherin-24) mediates strong cell-cell adhesion.
Cell membrane; Single-pass type I membrane protein.