Anti-Exportin T抗体(ab85365)

概述

  • 产品名称
    Anti-Exportin T抗体
  • 描述
    兔多克隆抗体to Exportin T
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from the N-terminal region of Human Exportin T (NP_009166), conjugated to KLH

  • 阳性对照
    • Ramos cell line lysate

性能

应用

Our Abpromise guarantee covers the use of ab85365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 110 kDa.
IHC-P 1/50 - 1/100.

靶标

  • 相关性
    This gene encodes a protein belonging to the RAN GTPase exportin family that mediates export of tRNA from the nucleus to the cytoplasm. Translocation of tRNA to the cytoplasm occurs once exportin has bound both tRNA and GTP bound RAN.
  • 细胞定位
    Nuclear; once bound to tRNA and Ran the complex translocates to the cytoplasm. Shuttles between the nucleus and the cytoplasm.
  • 数据库链接
  • 别名
    • 1110004L07Rik antibody
    • 3110065H13Rik antibody
    • AI452076 antibody
    • C79645 antibody
    • Exportin tRNA antibody
    • Exportin(tRNA) antibody
    • Nuclear export receptor for tRNAs antibody
    • tRNA exportin antibody
    • XPO3 antibody
    • XPOT antibody
    see all

图片

  • Anti-Exportin T antibody (ab85365) at 1/100 dilution + Human brain tissue lysate at 50 µg

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/8000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 110 kDa
    Observed band size : 110 kDa


    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-Exportin T antibody (ab85365) at 1/50 dilution + Ramos cell line lysates at 35 µg

    Predicted band size : 110 kDa
    Observed band size : 110 kDa
    Additional bands at : <55 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical staining of formalin-fixed and paraffin-embedded human lymph by ab85365 followed by staining with a HRP-conjugated secondary antibody and DAB staining.

文献

ab85365 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
ELISA
Sample
Human Tissue sections (BRAIN)
Specification
BRAIN
Type
Indirect
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Dec 06 2013

Application
Immunoprecipitation
Immuno-precipitation step
Other - Dynabead IgG
Sample
Human Tissue lysate - whole (BRAIN)
Specification
BRAIN
Total protein in input
500 µg
Username

Abcam user community

Verified customer

提交于 Dec 06 2013

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Human Tissue lysate - whole (brain)
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Dec 03 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册