概述

  • 产品名称Anti-Exosome Component 9抗体
    参阅全部 Exosome Component 9 一抗
  • 描述
    山羊多克隆抗体to Exosome Component 9
  • 特异性ab85703 is expected to recognize both reported isoforms (NP_001029366.1; NP_005024.2).
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    C-RTQTTSAKQEKAP

    , corresponding to C terminal amino acids 412-424 of Human Exosome Component 9 (NP_005024.2).

  • 阳性对照
    • Jurkat cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab85703 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 22262661
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 51 kDa). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Alderuccio, J Exp Med. 1991 Apr 1;173(4):941-52; PMID: 2007859).

靶标

  • 功能Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC9 binds to ARE-containing RNAs.
  • 序列相似性Belongs to the RNase PH family.
  • 细胞定位Nucleus > nucleolus; Cytoplasm. Nucleus > nucleolus and Nucleus. Excluded from the nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Autoantigen PM/Scl 1 antibody
    • EXOS9_HUMAN antibody
    • EXOSC 9 antibody
    • EXOSC9 antibody
    • Exosome complex component RRP45 antibody
    • Exosome complex exonuclease RRP45 antibody
    • Exosome component 9 antibody
    • p5 antibody
    • p6 antibody
    • P75 polymyositis scleroderma overlap syndrome associated autoantigen antibody
    • P75 polymyositis-scleroderma overlap syndrome-associated autoantigen antibody
    • PM/Scl 75 antibody
    • PM/Scl-75 antibody
    • PMSCL autoantigen 75kD antibody
    • PMSCL1 antibody
    • Polymyositis/scleroderma autoantigen 1 75kDa antibody
    • Polymyositis/scleroderma autoantigen 1 antibody
    • Polymyositis/scleroderma autoantigen 75 kDa antibody
    • RRP45 antibody
    • Rrp45p antibody
    see all

Anti-Exosome Component 9 antibody 图像

  • Anti-Exosome Component 9 antibody (ab85703) at 0.3 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 51 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

Anti-Exosome Component 9 antibody (ab85703)参考文献

This product has been referenced in:
  • Pfeiffer JR & Brooks SA Cullin 4B Is Recruited to Tristetraprolin-Containing Messenger Ribonucleoproteins and Regulates TNF-a mRNA Polysome Loading. J Immunol 188:1828-39 (2012). ICC/IF . Read more (PubMed: 22262661) »

See 1 Publication for this product

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