• 产品名称Anti-EXOSC8抗体
    参阅全部 EXOSC8 一抗
  • 描述
    兔多克隆抗体to EXOSC8
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide selected from the C terminal region of human EXOSC8.

  • 阳性对照
    • HL60 cell line lysates.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Ammonium Sulphate Precipitation
  • 纯化说明This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab82290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Predicted molecular weight: 30 kDa.
ELISA 1/1000.


  • 功能Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC8 binds to ARE-containing RNAs.
  • 序列相似性Belongs to the RNase PH family.
  • 细胞定位Cytoplasm. Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA421P11.3 antibody
    • CBP interacting protein 3 antibody
    • CIP3 antibody
    • EAP2 antibody
    • EC 3.1.13 antibody
    • EXOS8_HUMAN antibody
    • EXOSC8 antibody
    • Exosome complex component RRP43 antibody
    • Exosome complex exonuclease RRP43 antibody
    • Exosome component 8 antibody
    • OIP-2 antibody
    • OIP2 antibody
    • Opa interacting protein 2 antibody
    • Opa-interacting protein 2 antibody
    • OTTHUMP00000042274 antibody
    • p9 antibody
    • Ribosomal RNA processing protein 43 antibody
    • Ribosomal RNA processing protein 43, S. cerevisiae, homolog of antibody
    • Ribosomal RNA-processing protein 43 antibody
    • RP11 421P11.3 antibody
    • RRP43 antibody
    • Rrp43p antibody
    see all

Anti-EXOSC8 antibody 图像

  • Anti-EXOSC8 antibody (ab82290) at 1/50 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 30 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunofluorescence analysis of Human skin fibroblasts, staining EXOSC8 with ab82290.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1 % Triton-X-100 and blocked with 5% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 5% serum) for 3 hours at 25°C. An Cy2®-conjugated donkey anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.

    See Abreview

Anti-EXOSC8 antibody (ab82290)参考文献

ab82290 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - other (Human skin fibroblast)
Loading amount 40 µg
Specification Human skin fibroblast
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Dec 21 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Skin Fibroblasts)
Specification Skin Fibroblasts
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Dec 13 2012