概述

  • 产品名称Anti-EXOSC5抗体
    参阅全部 EXOSC5 一抗
  • 描述
    小鼠多克隆抗体to EXOSC5
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Full length protein (Human), EXOSC5 (AAH07742.1).

  • 阳性对照
    • human kidney tissue lysate, EXOSC5 transfected 293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 25 kDa).

靶标

  • 功能Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.
  • 组织特异性Highly expressed in a variety of hematopoietic and epithelial tumor cell lines, but not in normal hematopoietic tissues or other normal tissue, with the exception of testis.
  • 序列相似性Belongs to the RNase PH family.
  • 细胞定位Nucleus > nucleolus. Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Chronic myelogenous leukemia tumor antigen 28 antibody
    • CML28 antibody
    • EXOS5_HUMAN antibody
    • EXOSC 5 antibody
    • Exosc5 antibody
    • Exosome complex component rrp46 antibody
    • exosome complex exonuclease RRP46 antibody
    • Exosome component 5 antibody
    • exosome component Rrp46 antibody
    • hRrp46p antibody
    • p12B antibody
    • Ribosomal RNA processing protein 46 antibody
    • Ribosomal RNA processing protein 46, S. cerevisiae, homolog of antibody
    • Ribosomal RNA-processing protein 46 antibody
    • RRP41B antibody
    • RRP46 antibody
    • Rrp46p antibody
    see all

Anti-EXOSC5 antibody 图像

  • Anti-EXOSC5 antibody (ab69699) at 1/500 dilution + human kidney lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 25 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-EXOSC5 antibody (ab69699) at 1/500 dilution

    Lane 1 : EXOSC5 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 25 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
  • ab69699 at 10µg/ml staining EXOSC5 in human Hela cells by ICC/IF.

Anti-EXOSC5 antibody (ab69699)参考文献

This product has been referenced in:
  • Cammas A  et al. Destabilization of nucleophosmin mRNA by the HuR/KSRP complex is required for muscle fibre formation. Nat Commun 5:4190 (2014). Read more (PubMed: 24969639) »
  • Basu U  et al. The RNA Exosome Targets the AID Cytidine Deaminase to Both Strands of Transcribed Duplex DNA Substrates. Cell 144:353-63 (2011). WB ; Human, Mouse . Read more (PubMed: 21255825) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab69699.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"