Anti-EXOSC10/RRP6抗体(ab50558)
Key features and details
- Rabbit polyclonal to EXOSC10/RRP6
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-EXOSC10/RRP6抗体
参阅全部 EXOSC10/RRP6 一抗 -
描述
兔多克隆抗体to EXOSC10/RRP6 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Xenopus laevis, Pufferfish, Zebrafish, Cynomolgus monkey -
免疫原
Synthetic peptide corresponding to EXOSC10/RRP6 aa 231-245 (internal sequence) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Sequence:LDVPPALADFIHQQR-C
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50558于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa). Minor additional bands may be detected in some cell preparations.
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说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa). Minor additional bands may be detected in some cell preparations. |
靶标
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功能
Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA. -
序列相似性
Contains 1 3'-5' exonuclease domain.
Contains 1 HRDC domain. -
细胞定位
Cytoplasm. Nucleus, nucleolus. Nucleus. Strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. - Information by UniProt
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数据库链接
- Entrez Gene: 5394 Human
- Omim: 605960 Human
- SwissProt: Q01780 Human
- Unigene: 632368 Human
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别名
- Autoantigen PM/Scl 2 antibody
- Exosc10 antibody
- Exosome component 10 antibody
see all
图片
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Lanes 1-2 : Anti-EXOSC10/RRP6 antibody (ab50558) at 1 µg/ml
Lane 3 : Anti-EXOSC10/RRP6 antibody (ab50558) at 2 µg/ml
Lanes 1 & 3 : HeLa nuclear extract
Lane 2 : HeLa nuclear extract with PM/Scl-100 peptide
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate with a chemiluminescent substrate.
Predicted band size: 101 kDa
Observed band size: 101 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab50558 被引用在 25 文献中.
- Sun L et al. PD-L1 promotes myofibroblastic activation of hepatic stellate cells by distinct mechanisms selective for TGF-β receptor I versus II. Cell Rep 38:110349 (2022). PubMed: 35139382
- Cheng Y et al. N6-Methyladenosine on mRNA facilitates a phase-separated nuclear body that suppresses myeloid leukemic differentiation. Cancer Cell 39:958-972.e8 (2021). PubMed: 34048709
- Tanu T et al. hnRNPH1-MTR4 complex-mediated regulation of NEAT1v2 stability is critical for IL8 expression. RNA Biol 18:537-547 (2021). PubMed: 34470577
- Kawamoto T et al. ISG20 and nuclear exosome promote destabilization of nascent transcripts for spliceosomal U snRNAs and U1 variants. Genes Cells 26:18-30 (2021). PubMed: 33147372
- Green ID et al. Macrophage development and activation involve coordinated intron retention in key inflammatory regulators. Nucleic Acids Res N/A:N/A (2020). PubMed: 32449925