Anti-Exonuclease 1抗体(ab77375)

概述

  • 产品名称Anti-Exonuclease 1抗体
    参阅全部 Exonuclease 1 一抗
  • 描述
    山羊多克隆抗体to Exonuclease 1
  • 特异性ab77375 is expected to recognise all three reported isoforms (NP_003677.3; NP_006018.3 and NP_569082.1).
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    C-HRNYSPRPESGT

    , corresponding to internal sequence amino acids 372-383 of Human Exonuclease 1 (NP_003677.3; NP_006018.3; NP_569082.1).

  • 阳性对照
    • HEK293 and A549 cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab77375 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明Peptide ELISA: Antibody detection limit dilution 1/64000.
    WB: Use at a concentration of 0.2 - 0.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 94 kDa).
    An additional band of unknown identity was also consistently observed at 38kDa. This band was successfully blocked by incubation with the immunising peptide


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
    • 组织特异性Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
    • 序列相似性Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
    • 发展阶段Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.
    • 翻译后修饰Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
    • 细胞定位Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • exo1 antibody
      • EXO1_HUMAN antibody
      • ExoI antibody
      • Exonuclease 1 antibody
      • Exonuclease I antibody
      • Exonuclease1 antibody
      • HEX1 antibody
      • hExo I antibody
      • hExo1 antibody
      • hExoI antibody
      • Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1 antibody
      see all

    Anti-Exonuclease 1 antibody 图像

    • Anti-Exonuclease 1 antibody (ab77375) at 0.2 µg/ml + A549 cell lysate (in RIPA buffer) at 35 µg

      Predicted band size : 94 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.

    Anti-Exonuclease 1 antibody (ab77375)参考文献

    ab77375 has not yet been referenced specifically in any publications.

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