Anti-Exonuclease 1抗体(ab106303)

概述

  • 产品名称Anti-Exonuclease 1抗体
    参阅全部 Exonuclease 1 一抗
  • 描述
    兔多克隆抗体to Exonuclease 1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit, Chicken, Cow, Pig, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Exonuclease 1.

  • 阳性对照
    • WB: Human bone marrow, testis and thymus tissue lysates and HeLa, HEK293, A549 and A431 whole cell lysates. ICC/IF: HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab106303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/250. Detects a band of approximately 115 kDa (predicted molecular weight: 94 kDa).

靶标

  • 功能5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.
  • 组织特异性Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.
  • 序列相似性Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.
  • 发展阶段Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus.
  • 翻译后修饰Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.
  • 细胞定位Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase.
  • Information by UniProt
  • 数据库链接
  • 别名
    • exo1 antibody
    • EXO1_HUMAN antibody
    • ExoI antibody
    • Exonuclease 1 antibody
    • Exonuclease I antibody
    • Exonuclease1 antibody
    • HEX1 antibody
    • hExo I antibody
    • hExo1 antibody
    • hExoI antibody
    • Rad2 nuclease family member homolog of S. cerevisiae exonuclease 1 antibody
    see all

Anti-Exonuclease 1 antibody 图像

  • All lanes : Anti-Exonuclease 1 antibody (ab106303) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 6 : Human testis tissue lysate - total protein (ab30257)
    Lane 7 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 94 kDa
    Observed band size : 115 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 41 kDa,49 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • All lanes : Anti-Exonuclease 1 antibody (ab106303) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
    Lane 6 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 94 kDa


    Exposure time : 20 minutes
  • ICC/IF image of ab106303 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106303, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa cells at 5µg/ml.

Anti-Exonuclease 1 antibody (ab106303)参考文献

ab106303 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample African Green Monkey Cell lysate - whole cell (CV-1)
Loading amount 30 µg
Specification CV-1
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Oct 16 2012

DISCOUNT CODE: 100%ABR-T7KIT

Ablaufdatum: 20 Oktober 2012


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