The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/16000.
IHC-P: Use at a concentration of 5 - 10 µg/ml.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 74 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion.
Belongs to the EXO70 family.
The N-terminus is involved in SEC8 and ARHQ binding. The C-terminus is required for translocation to the plasma membrane.
Cytoplasm > cytosol. Cell membrane. Translocates, as a preformed complex with SEC6 and SEC8, to the plasma membrane in response to insulin through the activation of ARHQ.
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human adrenal gland tissue, using ab77352 at 5µg/ml. Note the staining of cytoplasm membranes and cytoplasm of secretory cells in the medulla.