The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 83 kDa.
Use a concentration of 3 µg/ml.
Use at an assay dependent concentration. PubMed: 21566143
Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. PubMed: 21108927
功能Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion.
序列相似性Belongs to the EXO70 family.
结构域The N-terminus is involved in SEC8 and ARHQ binding. The C-terminus is required for translocation to the plasma membrane.
细胞定位Cytoplasm > cytosol. Cell membrane. Translocates, as a preformed complex with SEC6 and SEC8, to the plasma membrane in response to insulin through the activation of ARHQ.
EXOC7 antibody (ab57402) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
Western blot - EXOC7 antibody (ab57402)
Predicted band size : 83 kDa EXOC7 antibody (ab57402) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Flow Cytometry - Anti-EXOC7 antibody (ab57402)
Overlay histogram showing HeLa cells stained with ab57402 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57402, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Anti-EXOC7 antibody (ab57402)参考文献
This product has been referenced in:
Hubert T et al. Actin and Arp2/3 localize at the centrosome of interphase cells. Biochem Biophys Res Commun404:153-8 (2011).
Read more (PubMed: 21108927) »
Barkefors I et al. Exocyst Complex Component 3-like 2 (EXOC3L2) Associates with the Exocyst Complex and Mediates Directional Migration of Endothelial Cells. J Biol Chem286:24189-99 (2011).
Read more (PubMed: 21566143) »