概述

  • 产品名称Anti-EXOC6B抗体
    参阅全部 EXOC6B 一抗
  • 描述
    兔多克隆抗体to EXOC6B
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C-terminal amino acids 766-796 of Human EXOC6B (NP_056004.1).

  • 阳性对照
    • Mouse heart tissue lysate IF/ICC: Hela cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab116383 was purified through a Protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab116383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 94 kDa.
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-EXOC6B antibody 图像

  • ab116383 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab116383 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-EXOC6B antibody (ab116383) at 1/100 dilution + Mouse heart tissue lysate at 35 µg

    Predicted band size : 94 kDa

Anti-EXOC6B antibody (ab116383)参考文献

This product has been referenced in:
  • Wen J  et al. Phenotypic and functional consequences of haploinsufficiency of genes from exocyst and retinoic acid pathway due to a recurrent microdeletion of 2p13.2. Orphanet J Rare Dis 8:100 (2013). WB . Read more (PubMed: 23837398) »

See 1 Publication for this product

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