概述

  • 产品名称Anti-EXO1抗体
  • 描述
    兔多克隆抗体to EXO1
  • 特异性This antibody has been shown to be specific for EXO1.
  • 经测试应用适用于: WB, Indirect ELISAmore details
  • 种属反应性
    与反应: Barley (Hordeum vulgare)
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 395-412 of Barley (Hordeum vulgare) EXO1

  • 阳性对照
    • Recombinant EXO1.
  • 常规说明For storage at 4°C add an appropriate antibacterial agent.

性能

应用

Our Abpromise guarantee covers the use of ab82533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000. Predicted molecular weight: 68 kDa.
Indirect ELISA 1/10000.

靶标

  • 相关性Family 3 beta-D-glucan glucohydrolases are widely distributed in higher plants. The enzymes catalyse the hydrolytic removal of beta-D-glucosyl residues from non-reducing termini of a range of beta-D-glucans and beta-D-oligoglucosides. Their broad specificity can be rationalized from X-ray crystallographic data obtained from a barley beta-D-glucan glucohydrolase in complex with non-hydrolysable S-glycoside substrate analogues, and from molecular modelling of enzyme-substrate complexes. The glucosyl residue occupying binding subsite -1 is tightly locked into a fixed position through extensive hydrogen bonding with six amino acid residues near the bottom of an active site pocket. In contrast, the glucosyl residue at subsite +1 is located between two tryptophan residues at the entrance of the pocket, where it is less tightly constrained. The relative flexibility of binding at subsite +1, coupled with the projection of the remainder of bound substrate away from the enzyme’s surface, means that the overall active site can accommodate a range of substrates with variable spatial dispositions of adjacent beta-D-glucosyl residues. The broad specificity for glycosidic linkage type would enable the enzyme to perform diverse functions during plant development.
  • 数据库链接
    • 别名
      • Beta D glucan exohydrolase isoenzyme ExoI antibody

    Anti-EXO1 antibody 图像

    • Lane 1 : Anti-EXO1 antibody (ab82533) at 1/5000 dilution
      Lane 2 : Pre-immune sera

      Lane 1 : Recombinant EXO1 (overloaded)
      Lane 2 : Recombinant EXO1 (overloaded)


      Predicted band size : 68 kDa
      Observed band size : 66 kDa (why is the actual band size different from the predicted?)

    Anti-EXO1 antibody (ab82533)参考文献

    ab82533 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab82533.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"