概述

  • 产品名称
  • 描述
    兔多克隆抗体to EVI1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within the internal amino acids 360-409 (HPSVGDNKPV ELQPERSSEE RPFEKISDQS ESSDLDDVST PSGSDLETTS) of Human EVI1, NP_005232.

  • 阳性对照
    • Human fetal brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab86306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 118 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性
    EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity. EVI1 may be involved in megakaryocytic differentiation.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • AML1 EVI 1 antibody
    • AML1 EVI 1 fusion protein antibody
    • AML1 EVI1 antibody
    • Ecotropic viral integration site 1 antibody
    • Ecotropic virus integration 1 site protein antibody
    • Ecotropic virus integration site 1 protein homolog antibody
    • EVI 1 antibody
    • EVI1 antibody
    • MDS1 and EVI1 complex locus antibody
    • MDS1 and EVI1 complex locus protein EVI1 antibody
    • MDS1 and EVI1 complex locus protein MDS1 antibody
    • MDS1 antibody
    • MDS1 EVI1 antibody
    • MGC163392 antibody
    • MGC97004 antibody
    • Myelodysplasia syndrome 1 antibody
    • Myelodysplasia syndrome 1 protein antibody
    • Myelodysplasia syndrome-associated protein 1 antibody
    • Oncogene EVI1 antibody
    • PRDM3 antibody
    • Zinc finger protein Evi1 antibody
    see all

图片

  • Anti-EVI1 antibody (ab86306) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 118 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab86306 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Mouse Cell lysate - whole cell (GM-CSF derived macrophages 8 days old)
Loading amount
15 µg
Specification
GM-CSF derived macrophages 8 days old
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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提交于 Jul 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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