Synthetic peptide corresponding to a region within the internal amino acids 360-409 (HPSVGDNKPV ELQPERSSEE RPFEKISDQS ESSDLDDVST PSGSDLETTS) of Human EVI1, NP_005232.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 118 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity. EVI1 may be involved in megakaryocytic differentiation.
- AML1 EVI 1 antibody
- AML1 EVI 1 fusion protein antibody
- AML1 EVI1 antibody
Western blot - Anti-EVI1 antibody (ab86306)
Anti-EVI1 antibody (ab86306) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
118 kDaObserved band size:
100 kDa (why is the actual band size different from the predicted?
Gel concentration 6-18%
has not yet been referenced specifically in any publications.
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