概述

  • 产品名称Anti-EVI1抗体
    参阅全部 EVI1 一抗
  • 描述
    兔多克隆抗体to EVI1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within the internal amino acids 360-409 (HPSVGDNKPV ELQPERSSEE RPFEKISDQS ESSDLDDVST PSGSDLETTS) of Human EVI1, NP_005232.

  • 阳性对照
    • Human fetal brain lysate

性能

应用

Our Abpromise guarantee covers the use of ab86306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 118 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity. EVI1 may be involved in megakaryocytic differentiation.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • AML1 EVI 1 antibody
    • AML1 EVI 1 fusion protein antibody
    • AML1 EVI1 antibody
    • Ecotropic viral integration site 1 antibody
    • Ecotropic virus integration 1 site protein antibody
    • Ecotropic virus integration site 1 protein homolog antibody
    • EVI 1 antibody
    • EVI1 antibody
    • MDS1 and EVI1 complex locus antibody
    • MDS1 and EVI1 complex locus protein EVI1 antibody
    • MDS1 and EVI1 complex locus protein MDS1 antibody
    • MDS1 antibody
    • MDS1 EVI1 antibody
    • MGC163392 antibody
    • MGC97004 antibody
    • Myelodysplasia syndrome 1 antibody
    • Myelodysplasia syndrome 1 protein antibody
    • Myelodysplasia syndrome-associated protein 1 antibody
    • Oncogene EVI1 antibody
    • PRDM3 antibody
    • Zinc finger protein Evi1 antibody
    see all

Anti-EVI1 antibody 图像

  • Anti-EVI1 antibody (ab86306) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 118 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

Anti-EVI1 antibody (ab86306)参考文献

ab86306 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (GM-CSF derived macrophages 8 days old)
Loading amount 15 µg
Specification GM-CSF derived macrophages 8 days old
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Jul 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"