Anti-EVER2抗体(ab69859)

概述

  • 产品名称Anti-EVER2抗体
    参阅全部 EVER2 一抗
  • 描述
    兔多克隆抗体to EVER2
  • 特异性At least three isoforms of EVER2 are known to exist; this antibody will only recognize the two larger isoforms. The antibody has no cross reactivity to EVER1.
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 14 amino acid synthetic peptide from near the center of human EVER2.

  • 阳性对照
    • Jurkat cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab69859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).Can be blocked with EVER2 peptide (ab169739).

靶标

  • 相关性Epidermodysplasia verruciformis (EV) is an autosomal recessive genodermatosis associated with a high risk of skin cancers resulting from a high susceptibility to infection by specific human papillomaviruses. Mutations in two homologous genes EVER1 and EVER2 cause the majority of EV cases. These two proteins form a complex and interact with the zinc transporter ZnT-1 in the endoplasmic reticulum. Cells lacking EVER2 accumulated higher levels of zinc in the nucleolus and nucleus compare to those cells with and intact EVER2 gene, indicating that one role of EVER2 is to regulate the intracellular distribution of zinc. At least two isoforms of EVER2 are known to exist.
  • 细胞定位Endoplasmic reticulum membrane; Multi pass membrane protein.
  • 数据库链接
  • 别名
    • epidermodysplasia verruciformis 2 antibody
    • Epidermodysplasia verruciformis protein 2 antibody
    • EV2 antibody
    • EVIN2 antibody
    • FLJ40668 antibody
    • FLJ43684 antibody
    • MGC102701 antibody
    • MGC40121 antibody
    • TMC8 antibody
    • Transmembrane channel like protein 8 antibody
    see all

Anti-EVER2 antibody 图像

  • Lane 1 : Anti-EVER2 antibody (ab69859) at 1 µg/ml
    Lane 2 : Anti-EVER2 antibody (ab69859) at 2 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 82 kDa
    Observed band size : 70,82 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.
  • ab69859 at 5 µg/ml staining EVER2 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • Immunofluorescence of EVER2 in Human Brain cells using ab69859 at 20 ug/ml.

Anti-EVER2 antibody (ab69859)参考文献

ab69859 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for ab67325 and ab69859 as it is proprietary information.

However, I can tell you that ab67325 is against a peptide from within xxxxx of ...

Read More

Thank you for providing your feedback on your experience with Abcam.

I am sorry to hear that the problem has not been solved.

We will replace the antibody for you. Please provide therefore the original order number of the antibody a...

Read More

Je suis désolée d'apprendre que ab69859 ne vous donne pas de bons résultats en Western blot.
Comme discuté au téléphone hier, j'avais contacté le laboratoire. Le laboratoire avait effectivement aussi vu des fois une bande additionnelle à 130+ kD d...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"