Anti-ETS1 (phospho T38)抗体(ab59179)

概述

  • 产品名称Anti-ETS1 (phospho T38)抗体
    参阅全部 ETS1 一抗
  • 描述
    兔多克隆抗体to ETS1 (phospho T38)
  • 特异性Detects endogenous levels of ETS1 only when phosphorylated at threonine 38.
  • 经测试应用适用于: IHC-P, ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide from around the phosphorylation site of threonine 38 (LLTPPS) of human ETS1

  • 阳性对照
    • Human breast carcinoma tissue. IF/ICC: HepG2 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab59179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).

靶标

  • 功能Transcription factor.
  • 序列相似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • 翻译后修饰Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
    Ubiquitinated; which induces proteasomal degradation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
    • C ets 1 protein antibody
    • ETS 1 antibody
    • Ets protein antibody
    • ETS1 antibody
    • ETS1 protein antibody
    • ETS1_HUMAN antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLJ10768 antibody
    • P54 antibody
    • Protein C-ets-1 antibody
    • v ets avian erythroblastosis virus E2 oncogene homolog antibody
    • v ets avian erythroblastosis virus E26 oncogene homolog 1 antibody
    • v ets erythroblastosis virus E26 oncogene homolog 1 antibody
    • v-ets erythroblastosis virus E26 oncogene homolog 1 antibody
    see all

Anti-ETS1 (phospho T38) antibody 图像

  • All lanes : Anti-ETS1 (phospho T38) antibody (ab59179) at 1/1000 dilution

    Lane 1 : Human A2058 whole cell lysate
    Lane 2 : Human A375 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Hourse anti-rabbit IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa (possible non-specific binding),180 kDa (possible non-specific binding),70 kDa (possible non-specific binding),90 kDa (possible non-specific binding).

    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ICC/IF image of ab59179 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59179, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab59179 at 1/50 dilution. Left: untreated. Right: sample treated with immunising peptide.

Anti-ETS1 (phospho T38) antibody (ab59179)参考文献

This product has been referenced in:
  • Selvaraj N  et al. Comparison of MAPK specificity across the ETS transcription factor family identifies a high-affinity ERK interaction required for ERG function in prostate cells. Cell Commun Signal 13:12 (2015). WB . Read more (PubMed: 25885538) »
  • Luo Y  et al. Transcription Factor Ets1 Regulates Expression of Thioredoxin-Interacting Protein and Inhibits Insulin Secretion in Pancreatic ß-Cells. PLoS One 9:e99049 (2014). Mouse . Read more (PubMed: 24897113) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12% SDS-PAGE)
Sample Human Cell lysate - whole cell (A2058 and A375)
Specification A2058 and A375
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

提交于 Dec 16 2013

Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab59179 has never been tested in Western blot. All tested applications covered by the 6 month guarantee are specified on our datasheets, and these are updated as ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"