Anti-ETS1抗体[mAbcam63302] (ab63302)

概述

性能

应用

Our Abpromise guarantee covers the use of ab63302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

靶标

Anti-ETS1 antibody [mAbcam63302] 图像

  • All lanes : Anti-ETS1 antibody [mAbcam63302] (ab63302) at 10 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 55,60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa (possible isoform).

    Exposure time : 2 minutes

Anti-ETS1 antibody [mAbcam63302] (ab63302)参考文献

ab63302 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 32 µg
Gel Running Conditions Reduced Denaturing (10% gel, semi-dry transfer)
Sample Chicken Cell lysate - whole cell (LMH cells overexpressing chicken Ets1A and B)
Specification LMH cells overexpressing chicken Ets1A and B
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Feb 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"