Anti-ETS1抗体[mAbcam63302] (ab63302)

概述

  • 产品名称
    Anti-ETS1抗体[mAbcam63302]
    参阅全部 ETS1 一抗
  • 描述
    小鼠单克隆抗体[mAbcam63302] to ETS1
  • 宿主
    Mouse
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Cow, Pig
  • 免疫原

    Recombinant fragment derived from within residues 100 - 200 of Human ETS1.

  • 阳性对照
  • 常规说明

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

性能

应用

Our Abpromise guarantee covers the use of ab63302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

靶标

  • 功能
    Transcription factor.
  • 序列相似性
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • 翻译后修饰
    Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
    Ubiquitinated; which induces proteasomal degradation.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
    • C ets 1 protein antibody
    • c-ets-1 antibody
    • ETS 1 antibody
    • Ets protein antibody
    • ETS proto-oncogene 1, transcription factor antibody
    • ETS1 antibody
    • ETS1 oncogene antibody
    • ETS1 protein antibody
    • ETS1_HUMAN antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLJ10768 antibody
    • Oncogene ETS1 antibody
    • P54 antibody
    • Protein C-ets-1 antibody
    • v ets avian erythroblastosis virus E2 oncogene homolog antibody
    • v ets avian erythroblastosis virus E2 oncogene homolog 1 antibody
    • v ets avian erythroblastosis virus E26 oncogene homolog 1 antibody
    • v ets erythroblastosis virus E26 oncogene homolog 1 antibody
    • v-ets erythroblastosis virus E26 oncogene homolog 1 antibody
    see all

图片

  • All lanes : Anti-ETS1 antibody [mAbcam63302] (ab63302) at 10 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 55,60 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 45 kDa (possible isoform)


    Exposure time: 2 minutes


    Ab63302 is predicted to react with both isoforms of ETS1. c-ETS-1A has a predicted molecular weight of 50-kDa, whereas c-ETS-1B has a predicted molecular weight of 41-kDa. The ETS1 protein sequence contains several phosphorylation sites (Swissprot), which might explain the banding pattern observed.

实验方案

文献

ab63302 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
32 µg
Gel Running Conditions
Reduced Denaturing (10% gel, semi-dry transfer)
Sample
Chicken Cell lysate - whole cell (LMH cells overexpressing chicken Ets1A and B)
Specification
LMH cells overexpressing chicken Ets1A and B
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Feb 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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