小鼠单克隆抗体[mAbcam63302] to ETS1
Mouse, Rat, Rabbit, Chicken, Cow, Pig
Recombinant fragment derived from within residues 100 - 200 of Human ETS1.
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact
firstname.lastname@example.org or you can find further information here.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.
Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
Ubiquitinated; which induces proteasomal degradation.
Information by UniProt
Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
C ets 1 protein antibody
Western blot - ETS1 antibody (ab63302)
All lanes :
Anti-ETS1 antibody [mAbcam63302] (ab63302) at 10 µg/ml
Lane 1 :
HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate
Lane 2 : Jurkat nuclear extract lysate ( ab14844) Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed ( ab97040) at 1/5000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa Observed band size: 55,60 kDa ( why is the actual band size different from the predicted?) Additional bands at: 45 kDa (possible isoform) Exposure time: 2 minutes Ab63302 is predicted to react with both isoforms of ETS1. c-ETS-1A has a predicted molecular weight of 50-kDa, whereas c-ETS-1B has a predicted molecular weight of 41-kDa. The ETS1 protein sequence contains several phosphorylation sites (Swissprot), which might explain the banding pattern observed.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"