概述

  • 产品名称Anti-ETS1抗体[EPR546(2)]
    参阅全部 ETS1 一抗
  • 描述
    兔单克隆抗体[EPR546(2)] to ETS1
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human ETS1.

  • 阳性对照
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 50 kDa).
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能Transcription factor.
    • 序列相似性Belongs to the ETS family.
      Contains 1 ETS DNA-binding domain.
      Contains 1 PNT (pointed) domain.
    • 翻译后修饰Sumoylated on Lys-15 and Lys-227, preferentially by SUMO2; which inhibits transcriptional activity.
      Ubiquitinated; which induces proteasomal degradation.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Avian erythroblastosis virus E26 (v ets) oncogene homolog 1 antibody
      • C ets 1 protein antibody
      • ETS 1 antibody
      • Ets protein antibody
      • ETS1 antibody
      • ETS1 protein antibody
      • ETS1_HUMAN antibody
      • EWSR 2 antibody
      • EWSR2 antibody
      • FLJ10768 antibody
      • P54 antibody
      • Protein C-ets-1 antibody
      • v ets avian erythroblastosis virus E2 oncogene homolog antibody
      • v ets avian erythroblastosis virus E26 oncogene homolog 1 antibody
      • v ets erythroblastosis virus E26 oncogene homolog 1 antibody
      • v-ets erythroblastosis virus E26 oncogene homolog 1 antibody
      see all

    Anti-ETS1 antibody [EPR546(2)] 图像

    • Overlay histogram showing Jurkat cells stained with ab109212 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109212, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-ETS1 antibody [EPR546(2)] (ab109212) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : Daudi cell lysate
      Lane 3 : Molt-4 cell lysate
      Lane 4 : PBMC lysate
      Lane 5 : HepG2 cell lysate
      Lane 6 : Jurkat cell lysate
      Lane 7 : Saos-2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 50 kDa

    Anti-ETS1 antibody [EPR546(2)] (ab109212)参考文献

    This product has been referenced in:
    • Luo W  et al. A balance between B cell receptor and inhibitory receptor signaling controls plasma cell differentiation by maintaining optimal Ets1 levels. J Immunol 193:909-20 (2014). Read more (PubMed: 24929000) »

    See 1 Publication for this product

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