Anti-ETFB抗体(ab73986)
Key features and details
- Rabbit polyclonal to ETFB
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ETFB抗体
参阅全部 ETFB 一抗 -
描述
兔多克隆抗体to ETFB -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide derived from the C-terminal domain of human ETFB
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73986于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/50 - 1/100.
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说明 |
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IHC-P
1/50 - 1/100. |
靶标
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功能
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). -
组织特异性
Abundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas. -
疾病相关
Glutaric aciduria 2B -
序列相似性
Belongs to the ETF beta-subunit/FixA family. -
结构域
The recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface. -
翻译后修饰
Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 2109 Human
- Entrez Gene: 110826 Mouse
- Entrez Gene: 292845 Rat
- Omim: 130410 Human
- SwissProt: P38117 Human
- SwissProt: Q9DCW4 Mouse
- SwissProt: Q68FU3 Rat
- Unigene: 348531 Human
see all -
别名
- Beta ETF antibody
- Beta-ETF antibody
- Electron transfer flavoprotein beta polypeptide antibody
see all
图片
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IHC image of ab73986 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73986, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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Datasheet download
文献 (2)
ab73986 被引用在 2 文献中.
- Schmiesing J et al. Interaction of glutaric aciduria type 1-related glutaryl-CoA dehydrogenase with mitochondrial matrix proteins. PLoS One 9:e87715 (2014). WB . PubMed: 24498361
- Codarin E et al. Differential proteomic analysis of subfractioned human hepatocellular carcinoma tissues. J Proteome Res 8:2273-84 (2009). PubMed: 19290626