概述

  • 产品名称Anti-ETFA抗体[2B11AE8]
    参阅全部 ETFA 一抗
  • 描述
    小鼠单克隆抗体[2B11AE8] to ETFA
  • 经测试应用适用于: IP, WB, ICC/IF, In-Cell ELISA, Flow Cyt, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Human recombinant full length protein ETFA

  • 阳性对照
    • HeLa cells Human heart tissue HepG2 whole cells Human liver mitochondria Bovine heart mitochondria Rat liver mitochondria Mouse liver mitochondria
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.
ICC/IF Use a concentration of 4 µg/ml.
In-Cell ELISA Use a concentration of 4 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
  • 疾病相关Glutaric aciduria 2A
  • 序列相似性Belongs to the ETF alpha-subunit/FixB family.
  • 结构域Domain I shares an identical polypeptide fold with the beta subunit ETFB though there is no sequence similarity.
  • 翻译后修饰The N-terminus is blocked.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha ETF antibody
    • Alpha-ETF antibody
    • Electron transfer flavoprotein alpha polypeptide antibody
    • Electron transfer flavoprotein alpha subunit antibody
    • Electron transfer flavoprotein subunit alpha mitochondrial antibody
    • Electron transfer flavoprotein subunit alpha, mitochondrial antibody
    • Electron transferring flavoprotein alpha polypeptide antibody
    • EMA antibody
    • ETFA antibody
    • ETFA_HUMAN antibody
    • GA2 antibody
    • Glutaric aciduria II antibody
    see all

Anti-ETFA antibody [2B11AE8] 图像

  • Immunocytochemistry image of stained HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the antibody (ab110316, 4 µg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix
  • All lanes : Anti-ETFA antibody [2B11AE8] (ab110316) at 1 µg/ml

    Lane 1 : Human heart tissue
    Lane 2 : HepG2 whole cells
    Lane 3 : Human liver mitochondria
    Lane 4 : Bovine heart mitochondria
    Lane 5 : Rat liver mitochondria
    Lane 6 : Mouse liver mitochondria

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 35 kDa
  • HL-60 cells were stained with 1 µg/mL ab110316 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • IHC image of ETFA staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110316, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-ETFA antibody [2B11AE8] (ab110316)参考文献

This product has been referenced in:
  • Davoudi M  et al. Complex I function and supercomplex formation are preserved in liver mitochondria despite progressive complex III deficiency. PLoS One 9:e86767 (2014). WB ; Mouse . Read more (PubMed: 24466228) »
  • Pougovkina O  et al. Mitochondrial protein acetylation is driven by acetyl-CoA from fatty acid oxidation. Hum Mol Genet N/A:N/A (2014). Mouse . Read more (PubMed: 24516071) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab110316.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"