概述

  • 产品名称
  • 描述
    兔多克隆抗体to ETAA1
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    DMPELFPSKT AHVTDQKEIC TFNSKTVKNT SRANTSPDAR LGDSKVLQDL SSKTYDRELI DAEYRFSPNS NKSNK, corresponding to amino acids 398-469 of Human ETAA1 (Q9NY74).

  • 阳性对照
    • Human kidney tissue, Human cell line U-2 OS.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: fixation of cells in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

靶标

图片

  • ab122245, at a 1/400 dilution, staining ETAA1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab122245, at 4 µg/ml, staining ETAA1 in Human cell line U-2 OS by Immunofluorescence.
  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

文献

ab122245 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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