Anti-Estrogen Sulfotransferase抗体[EST.1] (ab3091)

概述

  • 产品名称Anti-Estrogen Sulfotransferase抗体[EST.1]
    参阅全部 Estrogen Sulfotransferase 一抗
  • 描述
    小鼠单克隆抗体[EST.1] to Estrogen Sulfotransferase
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Purified rat liver Estrogen Sulfotransferase.

  • 阳性对照
    • Rat liver tissue sections. Rat liver lysate.

性能

应用

Our Abpromise guarantee covers the use of ab3091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
IHC-P
WB
  • 应用说明Immunofluorescence / Western Blotting: assay dependent.
    Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 2 - 4µg/ml for 30 minutes at RT.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    By Western blot, this antibody detects a band of ~ 31 kDa, which corresponds to the predicted molecular weight of Estrogen Sulfotransferase.

    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
  • 靶标

    • 功能May control the level of the estrogen receptor by sulfurylating free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated.
    • 组织特异性Liver, intestine and at lower level in the kidney.
    • 序列相似性Belongs to the sulfotransferase 1 family.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EST antibody
      • EST-1 antibody
      • EST1 antibody
      • Estrogen sulfotransferase antibody
      • estrogen-preferring antibody
      • estrone sulfotransferase antibody
      • ST1E1 antibody
      • ST1E1_HUMAN antibody
      • STE antibody
      • Sulfotransferase 1E1 antibody
      • Sulfotransferase antibody
      • Sulfotransferase estrogen preferring antibody
      • SULT1E1 antibody
      see all

    Anti-Estrogen Sulfotransferase antibody [EST.1] (ab3091)参考文献

    ab3091 has not yet been referenced specifically in any publications.

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