Anti-Estrogen Related Receptor gamma抗体- ChIP Grade (ab16366)

概述

  • 产品名称Anti-Estrogen Related Receptor gamma抗体- ChIP Grade
    参阅全部 Estrogen Related Receptor gamma 一抗
  • 描述
    兔多克隆抗体to Estrogen Related Receptor gamma - ChIP Grade
  • 特异性This antibody detects human Estrogen Related Receptor gamma from transfected cells and shows no cross reactivity to ERR1.
  • 经测试应用适用于: ChIP, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    DYEAGQHMEDPRR

    , corresponding to amino acids 401-413 of Human Estrogen Related Receptor gamma. This sequence is completely conserved between human, mouse and rat. (Peptide available as ab39779.)

  • 阳性对照
    • COS7 or HEK293 transfected cells. Human brain, kidney or pancreas.

性能

应用

Our Abpromise guarantee covers the use of ab16366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).Can be blocked with Human Estrogen Related Receptor gamma peptide (ab39779). Representing Estrogen Related Receptor gamma from COS7 transfected cells.

靶标

  • 功能Orphan receptor that acts as transcription activator in the absence of bound ligand. Binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements.
  • 组织特异性Expressed in the heart, kidney, brain, lung, bone marrow, adrenal gland, trachea, spinal cord and thyroid gland.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 发展阶段Expressed at high levels in fetal brain and also in the fetal kidney, lung and liver.
  • 翻译后修饰Sumoylation on Lys-40 is enhanced by phosphorylation at Ser-45 and represses transcriptional activity.
    Phosphorylation on Ser-45 enhances sumoylation on Lys-40 thus repressing transcriptional activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp781L1617 antibody
    • ERR 3 antibody
    • ERR G2 antibody
    • ERR gamma 2 antibody
    • ERR gamma-2 antibody
    • ERR3 antibody
    • ERR3_HUMAN antibody
    • ERRG 2 antibody
    • ERRG2 antibody
    • ERRgamma antibody
    • ESRRG antibody
    • Estrogen receptor related protein 3 antibody
    • Estrogen receptor-related protein 3 antibody
    • Estrogen-related receptor gamma antibody
    • FLJ16023 antibody
    • KIAA0832 antibody
    • NR3B3 antibody
    • Nuclear receptor subfamily 3 group B member 3 antibody
    see all

Anti-Estrogen Related Receptor gamma antibody - ChIP Grade 图像

  • Lanes 1 - 2 : Anti-Estrogen Related Receptor gamma antibody - ChIP Grade (ab16366) at 1/500 dilution
    Lane 3 : Anti-Estrogen Related Receptor gamma antibody - ChIP Grade (ab16366) at 1/1000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Mouse kidney cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 51 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)

Anti-Estrogen Related Receptor gamma antibody - ChIP Grade (ab16366)参考文献

ab16366 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. ERR3 (ab16366) is epitope affinity purified, but it appears there are a few non-specific bands around the 54 kDa range, which is why this product is recommended for cells overexpressing the protein. ERR3 has been used ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"