Anti-Estrogen Related Receptor beta抗体(ab19331)

概述

性能

应用

Our Abpromise guarantee covers the use of ab19331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 18179891
WB Use a concentration of 2 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP Use at an assay dependent concentration.
ICC/IF 1/50 - 1/200.

靶标

  • 功能
    Nuclear receptor, may regulate ESR1 transcriptional activity.
  • 疾病相关
    Defects in ESRRB are the cause of deafness autosomal recessive type 35 (DFNB35) [MIM:608565]. DFNB35 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Err 2 antibody
    • ERR b antibody
    • ERR B2 antibody
    • ERR beta 2 antibody
    • ERR beta antibody
    • ERR beta-2 antibody
    • ERR-beta antibody
    • Err2 antibody
    • ERR2_HUMAN antibody
    • ERRB 2 antibody
    • ERRb antibody
    • ERRB2 antibody
    • ERRbeta 2 antibody
    • ERRbeta antibody
    • ESR L2 antibody
    • ESRL 2 antibody
    • ESRL2 antibody
    • Esrrb antibody
    • Estrogen receptor like 2 antibody
    • Estrogen receptor related 2 antibody
    • Estrogen receptor-like 2 antibody
    • Estrogen-related receptor beta antibody
    • Estrrb antibody
    • Nr3b2 antibody
    • Nuclear receptor ERRB2 antibody
    • Nuclear receptor subfamily 3 group B member 2 antibody
    • Orphan nuclear receptor antibody
    • Steroid hormone receptor ERR 2 antibody
    • Steroid hormone receptor ERR2 antibody
    see all

图片

  • Western blot detection of Estrogen Related Receptor beta from 100 ug of rat kidney lysate (1:500).

  • ab19331 staining Estrogen Related Receptor Beta protein on mouse embryonic stem cell by ICC/IF. The cells were fixed in 1:1 methanol:acetone and blocked in 0.1% BSA plus 1% serum, for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the cells for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rabbit IgG, diluted 1/200, was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Chaudhary S  et al. Overexpression of caspase 7 is ERa dependent to affect proliferation and cell growth in breast cancer cells by targeting p21(Cip). Oncogenesis 5:e219 (2016). ChIP . Read more (PubMed: 27089142) »
  • Kunst S  et al. Photoreceptor cells display a daily rhythm in the orphan receptor Esrrß. Mol Vis 21:173-84 (2015). WB . Read more (PubMed: 25737630) »

See all 3 Publications for this product

客户评价及客户问答

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic)
Permeabilization
Yes - Triton X-100 (0.5%)
Specification
Embryonic
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 22 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic stem cell)
Specification
embryonic stem cell
Fixative
methanol:acetone 1:1
Permeabilization
No
Blocking step
0.1%BSA and 1% serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Nov 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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