Anti-Estrogen Related Receptor alpha抗体- ChIP Grade (ab16363)

概述

  • 产品名称
    Anti-Estrogen Related Receptor alpha抗体- ChIP Grade
    参阅全部 Estrogen Related Receptor alpha 一抗
  • 描述
    兔多克隆抗体to Estrogen Related Receptor alpha - ChIP Grade
  • 特异性
    This antibody shows no cross-reactivity to ERR3.
  • 经测试应用
    适用于: IHC-P, IP, WB, ChIPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Dog
  • 免疫原

    Synthetic peptide:

    MSSQVVGIEPLYIKAE

    , corresponding to amino acids 1-16 of Human Estrogen Related Receptor alpha. This sequence is completely conserved between canine, human, mouse and rat. (Peptide available as ab39777.)

  • 阳性对照
    • Estrogen related receptor alpha transfected Cos-7 cells.

应用

Our Abpromise guarantee covers the use of ab16363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100.
IP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 52, 37 kDa (predicted molecular weight: 55 kDa).Can be blocked with Human Estrogen Related Receptor alpha peptide (ab39777).
ChIP Use 0.26 µg for µg of chromatin.

靶标

  • 功能
    Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 翻译后修饰
    Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
    Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Err 1 antibody
    • ERR a antibody
    • ERR alpha antibody
    • ERR-alpha antibody
    • Err1 antibody
    • ERR1 protein antibody
    • ERR1_HUMAN antibody
    • ERRa antibody
    • ERRalpha antibody
    • ESRL 1 antibody
    • ESRL1 antibody
    • ESRR A antibody
    • Esrra antibody
    • Estrogen receptor like 1 antibody
    • Estrogen receptor related 1 antibody
    • estrogen receptor related receptor alpha antibody
    • Estrogen receptor-like 1 antibody
    • Estrogen related receptor alpha antibody
    • Estrogen-related receptor alpha antibody
    • Estrra antibody
    • hERR1 antibody
    • NR3B1 antibody
    • Nuclear receptor subfamily 3 group B member 1 antibody
    • Steroid hormone receptor ERR1 antibody
    see all

Anti-Estrogen Related Receptor alpha antibody - ChIP Grade 图像

  • Detection of Estrogen Related Receptor alpha binding at ESRRA and ACADM promoters by ab16363 in ChIP. MCF7 breast epithelial adenocarcinoma cells were treated with estrogen or vehicle, followed by chromatin extraction and crosslinking. The ChIP was performed using ab16363 at a concentration of 0.26 µg/µg chromatin. The DNA was quantified using Real-time PCR. Please see abreview by Genpathway Inc for additional details.

    See Abreview

  • Estrogen Related Receptor alpha was immunoprecipitated using 0.5mg MCF7 whole cell extract, 5µg of Rabbit polyclonal to Estrogen Related Receptor alpha and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, MCF7 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16363.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Anti-Rabbit HRP (IgG light chain) (ab99697).
    Band: 48kDa: Estrogen Related Receptor alpha.

  • ab16363 staining Estrogen Related Receptor alpha in mouse heart tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using TRIS/EDTA pH 9.0. Samples were then blocked with 2% normal goat serum for 45 minutes at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. A biotin-conjugated rabbit polyclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview

Anti-Estrogen Related Receptor alpha antibody - ChIP Grade (ab16363)参考文献

This product has been referenced in:
  • Emmett MJ  et al. Histone deacetylase 3 prepares brown adipose tissue for acute thermogenic challenge. Nature 546:544-548 (2017). ChIP ; Mouse . Read more (PubMed: 28614293) »
  • Holloway KR  et al. SIRT1 positively regulates breast cancer associated human aromatase (CYP19A1) expression. Mol Endocrinol 27:480-90 (2013). ChIP ; Human . Read more (PubMed: 23340254) »

See all 5 Publications for this product

Product Wall

I am very pleased to hear you would like to accept our offer and test ab16363 in rat samples. This code will give you the VALUE of this product off an order before the expiration date. To redeem this offer, please submit an Abreview for rat samples. Th...

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Thank you very much for your interest in ab16363.

To our knowledge, this product has not been tested in rat. However as this sequence is completely conserved in rat, it is very likely that the product will work in rat. By participating in...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF-7)
Loading amount
50 µg
Specification
MCF-7
Gel Running Conditions
Reduced Denaturing (4-15)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ping Yi

Verified customer

提交于 Jul 20 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (placentome, uterus, small intestine)
Specification
placentome, uterus, small intestine
Fixative
Carnoy
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Buffers with pH 6, 9, 8
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Ms. Roza Yunusova

Verified customer

提交于 May 07 2012

Thank you for your phone call.


As we discussed over the phone, trying another antigen retrieval method (e.g. Tris EDTA) might help with the problem of no signal.

DISCOUNT CODE: xxx
Expiration date: xxx


I ...

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Thank you for your reply.
Below are the testing discount details for ab16363. As for ab97085, it is an HRP-tagged secondary antibody that will work with ab16363 and from your previous correspondence I did see that you were credited the cost of 2 a...

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Thank you very much for contacting Abcam.
To our knowledge, ab16363 has not been tested in sheep tissue. I cannot find the protein sequence for sheep ESRR, but as the homology between human, mouse, rat bovine and pig are all very close for this pr...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Blocking step
Normal Goat Serum as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Mrs. Marisa Mcdonald

Verified customer

提交于 Oct 19 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (MCF7 breast epithelial adenocarcinoma)
Specification
MCF7 breast epithelial adenocarcinoma
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
Region upstream of the transcription start site of the ESRRA gene
Region upstream of the transcription start site of the ACADM gene
Negative control
Region on chromosome 12 (Untr12) that is far from any known gene annotation and not expected to be bound by ESRRA
Username

Mr. Genpathway Inc

Verified customer

提交于 May 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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