Anti-Estrogen Receptor beta (phospho S105)抗体(ab62257)

概述

  • 产品名称Anti-Estrogen Receptor beta (phospho S105)抗体
    参阅全部 Estrogen Receptor beta 一抗
  • 描述
    兔多克隆抗体to Estrogen Receptor beta (phospho S105)
  • 特异性ab62257 detects endogenous levels of Estrogen Receptor beta only when phosphorylated at serine 105.
  • 经测试应用适用于: IHC-P, WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human Estrogen Receptor beta around the phosphorylation site of serine 105 (Q-K-SP-P-W).

  • 阳性对照
    • HuvEc cells.

性能

应用

Our Abpromise guarantee covers the use of ab62257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 20696772
WB Use at an assay dependent concentration. PubMed: 20696772
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
  • 组织特异性Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ER BETA antibody
    • ER-beta antibody
    • Erb antibody
    • ESR B antibody
    • ESR BETA antibody
    • ESR2 antibody
    • ESR2_HUMAN antibody
    • ESRB antibody
    • ESTRB antibody
    • estrogen nuclear receptor beta variant a antibody
    • estrogen nuclear receptor beta variant b antibody
    • estrogen receptor 2 (ER beta) antibody
    • Estrogen receptor 2 antibody
    • estrogen receptor beta 4 antibody
    • Estrogen receptor beta antibody
    • NR3A2 antibody
    • Nuclear receptor subfamily 3 group A member 2 antibody
    see all

Anti-Estrogen Receptor beta (phospho S105) antibody 图像

  • ab62257 at 1/500 - 1/1000 dilution staining Estrogen Receptor beta in HuvEc cells by Immunofluorescence, in the absence or presence of the immunising peptide

Anti-Estrogen Receptor beta (phospho S105) antibody (ab62257)参考文献

This product has been referenced in:
  • Hamilton-Burke W  et al. Phosphorylation of Estrogen Receptor {beta} at Serine 105 Is Associated with Good Prognosis in Breast Cancer. Am J Pathol 177:1079-86 (2010). WB, IHC-P ; Human . Read more (PubMed: 20696772) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab62257.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"