Anti-Estrogen Receptor beta抗体[EPR3777] (ab92306)


  • 产品名称Anti-Estrogen Receptor beta抗体[EPR3777]
    参阅全部 Estrogen Receptor beta 一抗
  • 描述
    兔单克隆抗体[EPR3777] to Estrogen Receptor beta
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to the N terminal of Human Estrogen Receptor beta was used as an immunogen (UniProt ID: Q92731).

  • 阳性对照
    • WB: MCF-7 and HeLa cell lysates. IP: HeLa cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:
    Anti-Estrogen Receptor beta antibody (HRP) [EPR3777] (ab200599)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.



Our Abpromise guarantee covers the use of ab92306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
IP 1/10 - 1/120.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • 靶标

    • 功能Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
    • 组织特异性Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
    • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ER BETA antibody
      • ER-beta antibody
      • Erb antibody
      • ESR B antibody
      • ESR BETA antibody
      • ESR2 antibody
      • ESR2_HUMAN antibody
      • ESRB antibody
      • ESTRB antibody
      • estrogen nuclear receptor beta variant a antibody
      • estrogen nuclear receptor beta variant b antibody
      • estrogen receptor 2 (ER beta) antibody
      • Estrogen receptor 2 antibody
      • estrogen receptor beta 4 antibody
      • Estrogen receptor beta antibody
      • NR3A2 antibody
      • Nuclear receptor subfamily 3 group A member 2 antibody
      see all

    Anti-Estrogen Receptor beta antibody [EPR3777] 图像

    • All lanes : Anti-Estrogen Receptor beta antibody [EPR3777] (ab92306) at 1/1000 dilution (purified)

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 59 kDa
      Observed band size : 59 kDa

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • ab92306 (purified) at 1/20 immunoprecipitating Estrogen Receptor beta in 10 μg HeLa cell lysate (Lanes 1 and 2, observed at 82-95 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/1000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    • All lanes : Anti-Estrogen Receptor beta antibody [EPR3777] (ab92306) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 59 kDa

    Anti-Estrogen Receptor beta antibody [EPR3777] (ab92306)参考文献

    ab92306 has not yet been referenced specifically in any publications.

    Product Wall

    We have not tested ab92306 for its potential cross-reactivity with estrogen receptor alpha. Sequence homology is 66.7% between the immunogen of ER beta and ER alpha, so we would not predict cross-reactivity. However, it is not guaranteed without testin...

    Read More